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Animal species milk identification by comparison of two-dimensional gel map profile and mass spectrometry approach

文献类型： 外文期刊

作者： Yang, Yongxin 1 ; Zheng, Nan 1 ; Yang, Jinhui 1 ; Bu, Dengpan 1 ; Wang, Jiaqi 1 ; Ma, Lu 1 ; Sun, Peng 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China

2.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Hefei 230031, Peoples R China

期刊名称：INTERNATIONAL DAIRY JOURNAL （ 影响因子：3.032； 五年影响因子：3.395 ）

ISSN： 0958-6946

年卷期： 2014 年 35 卷 1 期

页码：

收录情况： SCI

摘要： The aim of the current study was to present the primary protein profile of cow, goat, camel, yak and buffalo milk, along with binary mixtures of these milks through two-dimensional gel electrophoresis coupled with mass spectrometry for detection of specific milks in mixtures. Distributions of alpha-lactalbumin and/or beta-lactoglobulin spots on gel maps were used to detect goat, camel, yak and buffalo milk adulterated with cow milk. Appearance of alpha-lactalbumin and beta-lactoglobulin protein spots were helpful for detection of camel, yak and buffalo milk adulteration with goat milk. alpha(S1)-Casein from cow and goat milk was also used to determine camel milk adulteration. In particular, beta-lactoglobulin from cow, goat, yak and buffalo milk, and alpha-lactalbumin from camel milk were useful to detect adulteration of specific milk mixtures at levels as low as 0.5%. These results highlight applicability of this method for characterisation of milk proteome and detection of specific milk in mixtures. (C) 2013 Elsevier Ltd. All rights reserved.

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