Bovine colostrum promoted ileal health in newborn lambs at 24 h after birth: insight from intestinal morphology and innate immunity
文献类型: 外文期刊
作者: Zhu, Honglong 1 ; Yang, Yongxin 2 ; Wu, Tao 1 ; Qi, Yunxia 1 ; Huang, Dongwei 1 ; Han, Rongwei 2 ; Chen, Sheng 1 ; Tang, Jishun 1 ; Ren, Man 3 ; Zhao, Xiaowei 1 ;
作者机构: 1.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Anhui Prov Key Lab Livestock & Poultry Prod Safet, Hefei 230031, Anhui, Peoples R China
2.Qingdao Agr Univ, Coll Food Sci & Engn, Qingdao 266109, Peoples R China
3.Anhui Sci & Technol Univ, Coll Anim Sci, Anhui Prov Key Lab Anim Nutr Regulat & Hlth, Bangbu 233100, Peoples R China
关键词: Inflammatory cytokines; Intestinal barrier; Mucosa growth; Sheep; Toll-like receptors
期刊名称:ANIMAL ( 影响因子:3.73; 五年影响因子:3.908 )
ISSN: 1751-7311
年卷期: 2022 年 16 卷 8 期
页码:
收录情况: SCI
摘要: The contribution of colostrum to passive immunity transfer and intestinal protection in newborn ruminants is well known; however, it is currently unclear how colostrum intake affects intestinal innate immunity. We investigated the effects of bovine colostrum intake on ileal morphology, expression of genes involved in intestinal innate immunity, and serum concentrations of inflammatory cytokines in newborn lambs. Twenty-seven newborn male Hu lambs were used, of which 18 were bottle-fed either bovine colostrum (C24h; n = 9) or bovine mature milk (M24h; n = 9) within the first 2 h after birth at an intake of approximately 8% of BW; the remaining nine lambs did not receive any feeding (N24h). Blood and ileal tissue samples were collected after the lambs were slaughtered at 24 h after birth. Ileal villus height and villus height-to-crypt depth ratio were significantly higher in C24h than those in N24h and M24h lambs (P < 0.01). Messenger RNA (mRNA) abundance of toll-like receptor (TLR)-2, TLR3, TLR4, TLR6, TLR7, TLR8 and tumour necrosis factor alpha in the ileum was lower in C24h than that in N24h lambs (P < 0.05). Moreover, C24h lambs had a lower TLR3 mRNA abundance (P < 0.01) and a trend of lower TLR6 (P = 0.06) and interleukin 1 beta (P = 0.08) expression compared with those in M24h lambs. We also observed strong positive correlations of tumour necrosis factor alpha expression with that of TLR2 (r = 0.71; P < 0.001), TLR4 (r = 0.88; P < 0.001) and TLR8 (r = 0.83; P < 0.001). Interestingly, the expression of barrier-related molecules, including mucin-13, lysozyme, claudin (CLDN)-1, CLDN2, CLDN4, CLDN7, CLDN12, occludin, zonula occluden-1 and junctional adhesion molecule-1, was consistently lower in C24h lambs than that in N24h and M24h lambs (P < 0.05). These results indicated that the beneficial roles of colostrum intake on intestinal protection in newborn lambs were associated with low TLR expression, which was reflected by improved intestinal development and reduced inflammatory response. Further studies using fluorescence in situ hybridisation and immunohistochemical methods are needed to further explore the mechanisms underlying the lower expression of intestinal barrier-related molecules due to colostrum feeding. (C) 2022 The Authors. Published by Elsevier B.V. on behalf of The Animal Consortium.
- 相关文献
作者其他论文 更多>>
-
Integration Analysis of Hair Follicle Transcriptome and Proteome Reveals the Mechanisms Regulating Wool Fiber Diameter in Angora Rabbits
作者:Huang, Dongwei;Ding, Haisheng;Wang, Yuanlang;Wang, Xiaofei;Zhao, Huiling
关键词:fiber diameter; hair follicles; integration analysis; proteomic; Angora rabbits
-
Rumen microbiota succession throughout the perinatal period and its association with postpartum production traits in dairy cows: A review
作者:Zhao, Xiaowei;Zhang, Yangdong;Rahman, Ashikur;Chen, Meiqing;Li, Ning;Zheng, Nan;Zhao, Shengguo;Wang, Jiaqi;Zhao, Xiaowei;Wu, Tao;Qi, Yunxia;Zhao, Xiaowei
关键词:Dairy cow; Perinatal period; Rumen microbiota; Production trait
-
Integrated 4D Analysis of Intramuscular Fat Deposition: Quantitative Proteomic and Transcriptomic Studies in Wannanhua Pig Longissimus Dorsi Muscle
作者:Li, Xiaojin;Xie, Fei;Li, Ruidong;Li, Lei;Ren, Man;Jin, Mengmeng;Li, Shenghe;Li, Xiaojin;Xie, Fei;Li, Ruidong;Li, Lei;Ren, Man;Jin, Mengmeng;Li, Shenghe;Xie, Fei;Wang, Chonglong;Zhou, Ju
关键词:Wannanhua pig; proteomics; IMF; transcriptomics; longissimus dorsi muscle; PRM
-
Key mRNAs and lncRNAs of pituitary that affect the reproduction of FecB plus plus small tail han sheep
作者:Yang, Jianqi;He, Xiaoyun;Di, Ran;Hu, Wenping;Chu, Mingxing;Tang, Jishun;Zhang, Xiaosheng;Zhang, Jinlong;Guo, Xiaofei
关键词:Pituitary; mRNAs; lncRNAs; Reproduction; Sheep
-
Comparative Transcriptomics Identify Key Pituitary Circular RNAs That Participate in Sheep (Ovis aries) Reproduction
作者:Yang, Jianqi;He, Xiaoyun;Di, Ran;Chu, Mingxing;Hu, Wenping;Tang, Jishun;Zhang, Xiaosheng;Zhang, Jinlong;Guo, Xiaofei
关键词:circular RNAs; pituitary; reproduction; sheep
-
Transcriptome analysis of divergent residual feed intake phenotypes in the M. longissimus thoracis et lumborum of Wannan Yellow rabbits
作者:Huang, Dongwei;Wang, Yuanlang;Qi, Pingping;Ding, Haisheng;Zhao, Huiling
关键词:feed efficiency; residual feed intake; rabbit production; transcriptome; differentially expressed gene; signaling pathway; M. longissimus thoracis et lumborum; meat quality
-
Hair Follicle Transcriptome Analysis Reveals Differentially Expressed Genes That Regulate Wool Fiber Diameter in Angora Rabbits
作者:Huang, Dongwei;Ding, Haisheng;Wang, Yuanlang;Cheng, Guanglong;Wang, Xiaofei;Leng, Taishan;Zhao, Huiling
关键词:wool fiber diameter; hair follicles; RNA-Seq; GSEA; PPI; hair structure