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Establishment of a survey method to determine rice stigma length from preparation, sampling, and preservation to measurement

文献类型： 外文期刊

作者： Jiang, Jianhua 1 ; Zhang, Wuhan 2 ; Lu, Chao 3 ; Xu, Lei 1 ; Hu, Changmin 1 ; Zhang, Ying 1 ; He, Qiang 2 ; Wang, Dezheng 1 ;

作者机构： 1.Anhui Acad Agr Sci, Anhui Key Lab Rice Genet & Breeding, Inst Rice Res, Hefei, Peoples R China

2.Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha, Peoples R China

3.Changde Acad Agr & Forestry Sci, Inst Rice Res, Changde, Peoples R China

关键词： development stage; fixative solution; measured; stigma length; rice

期刊名称：PLANT PRODUCTION SCIENCE （ 影响因子：2.222； 五年影响因子：2.175 ）

ISSN： 1343-943X

年卷期：

页码：

收录情况： SCI

摘要： The lack of a unified standard for sampling stage, preservation and measurement of stigma length is the main restriction factor for the study of stigma length trait in rice. In this study, stigma lengths were compared among four different developmental stages (T1-T4) of 30 rice materials under two environments (years) and in 10 measurement periods (D1-D120) of 3 rice materials after treatment with 6 fixatives (S1-S6), and finally the results revealed that the T3 were the best sampling stage and the S6 was the most suitable fixative for stigma length. This study thoroughly describes the process for surveying and measuring stigma length in 6 steps: preparation before sampling, flower sampling, focusing, flower dissection and imaging, obtaining measurements, and data analysis. These findings provide an outline of the methodology for the discovery, fine mapping, and functional analysis of the genes that control the stigma length trait. [GRAPHICS] .

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