文献类型： 外文期刊

作者： Lu, Xue-Mei ¹ ; Yu, Xiao-Fen ¹ ; Li, Guo-Qiang ² ; Qu, Ming-Hao ¹ ; Wang, Huan ⁴ ; Liu, Chuang ⁵ ; Man, Yu-Ping ¹ ; Jiang, Xiao-Han ¹ ; Li, Mu-Zi ¹ ; Wang, Jian ¹ ; Chen, Qi-Qi ¹ ; Lei, Rui ¹ ; Zhao, Cheng-Cheng ¹ ; Zhou, Yun-Qiu ¹ ; Jiang, Zheng-Wang ¹ ; Li, Zuo-Zhou ¹ ; Zheng, Shang ⁴ ; Dong, Chang ⁶ ; Wang, Bai-Lin ⁷ ; Sun, Yan-Xiang ⁸ ; Zhang, Hui-Qin ⁹ ; Li, Jie-Wei ¹ ; Mo, Quan-Hui ¹ ; Zhang, Ying ¹ ; Lou, Xin ¹ ; Peng, Hai-Xu ¹ ; Yi, Ya-Ting ¹ ; Wang, He-Xin ¹ ; Zhang, Xiu-Jun ¹ ; Wang, Yi-Bo ² ; Wang, Dan ¹⁴ ; Li, Li ¹ ; Zhang, Qiong ¹ ; Wang, Wen-Xia ¹⁵ ; Liu, Yongbo ¹⁶ ; Gao, Lei ¹ ; Wu, Jin-Hu ¹⁸ ; Wang, Yan-Chang ¹ ;

作者机构： 1.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Specialt, Wuhan Bot Garden, Wuhan, Hubei, Peoples R China

2.Lanzhou Univ, Coll Earth & Environm Sci, Key Lab Western Chinas Environm Syst, Minist Educ, Lanzhou, Gansu, Peoples R China

3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China

4.Wuhan Frasergen Bioinformat Co Ltd, Wuhan, Hubei, Peoples R China

5.Anhui Acad Agr Sci, Inst Soil & Fertilizer, Hefei, Peoples R China

6.Xichang Univ, Coll Agr Sci, Xichang, Sichuan, Peoples R China

7.Heilongjiang Acad Agr Sci, Hort Subacad, Harbin, Heilongjiang, Peoples R China

8.Langfang Normal Univ, Coll Life Sci, Langfang, Hebei, Peoples R China

9.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou, Zhejiang, Peoples R China

10.Chinese Acad Sci, Guangxi Inst Bot, Guilin, Guangxi, Peoples R China

11.Inst Bot Shaanxi Prov, Xian Bot Garden Shaanxi Prov, Xian, Shaanxi, Peoples R China

12.Dalian Univ, Inst Modern Agr Res, Dalian, Liaoning, Peoples R China

13.Beijing Univ Agr, Coll Plant Sci & Technol, Bioinformat Ctr, Beijing, Peoples R China

14.Eastern Liaoning Univ, Coll Agr, Dandong, Liaoning, Peoples R China

15.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Liaoning, Peoples R China

16.Chinese Res Inst Environm Sci, State Environm Protect Key Lab Reg Ecoproc & Funct, Beijing, Peoples R China

17.Hubei Hongshan Lab, Wuhan, Hubei, Peoples R China

18.Food Res Ltd, New Zealand Inst Plant, Auckland, New Zealand

关键词： kiwiberry; dioecism; adaptive evolution; paleoclimate; GEA; rapid softening

期刊名称：PLANT COMMUNICATIONS （ 影响因子：9.4； 五年影响因子：9.4 ）

ISSN： 2590-3462

年卷期： 2024 年 5 卷 6 期

页码：

收录情况： SCI

摘要： Actinidia arguta , the most widely distributed Actinidia species and the second cultivated species in the genus, can be distinguished from the currently cultivated Actinidia chinensis on the basis of its small and smooth fruit, rapid softening, and excellent cold tolerance. Adaptive evolution of tetraploid Actinidia species and the genetic basis of their important agronomic traits are still unclear. Here, we generated a chromosome -scale genome assembly of an autotetraploid male A. arguta accession. The genome assembly was 2.77 Gb in length with a contig N50 of 9.97 Mb and was anchored onto 116 pseudo -chromosomes. Resequencing and clustering of 101 geographically representative accessions showed that they could be divided into two geographic groups, Southern and Northern, which first diverged 12.9 million years ago. A. arguta underwent two prominent expansions and one demographic bottleneck from the midPleistocene climate transition to the late Pleistocene. Population genomics studies using paleoclimate data enabled us to discern the evolution of the species' adaptation to different historical environments. Three genes ( AaCEL1 , AaPME1 , and AaDOF1 ) related to flesh softening were identified by multi-omics analysis, and their ability to accelerate flesh softening was verified through transient expression assays. A set of genes that characteristically regulate sexual dimorphism located on the sex chromosome (Chr3) or autosomal chromosomes showed biased expression during stamen or carpel development. This chromosome -level assembly of the autotetraploid A. arguta genome and the genes related to important agronomic traits will facilitate future functional genomics research and improvement of A. arguta .