文献类型： 外文期刊
作者： Li, Qinggang 1 ; Tao, Zhu 1 ; Shi, Lihua 1 ; Ban, Dongmei 1 ; Zhang, Bo 1 ; Yang, Yuzeng 1 ; Zhang, Hao 1 ; Wu, Changxin 1 ;
作者机构： 1.China Agr Univ, Coll Anim Sci & Technol, Natl Engn Lab Anim Breeding, Beijing 100193, Peoples R China
2.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Hefei 230031, Anhui, Peoples R China
关键词： ACSL1;mRNA expression;Polymorphism;Adipose deposition;Pig
期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）
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收录情况： SCI
摘要： Acyl coenzyme A long-chain 1 synthetase (ACSL1) plays a key role in animal fat synthesis and fatty acid beta-oxidation. In order to research the function of the ACSL1 gene in pig, we analyzed the mRNA expression in liver, backfat and longissimus dorsi muscle by quantitative real-time PCR in Tibet pig (TP, n = 10), Diannan small ear pig (DSP, n = 10) and large white pig (LW, n = 10). The results showed that the mRNA expressions of the ACSL1 gene in liver and longissimus dorsi muscle of DSP and TP were significant higher than that of LW (P < 0.01). However, the expression in backfat of LW was significant higher than that of TP (P < 0.01) and DSP (P < 0.05). In addition, four SNPs located in 5' flanking region (T-1191C), exon 6(G173A), exon 14(C36T) and exon 17(T46C) were identified, and the allele frequencies of the four SNPs were significant different in indigenous and introduced pig breeds. The results indicated that the ACSL1 gene might be relative to the capacity of fat deposition and meat quality in pig breeds.
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