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A respiratory burst oxidase homolog protein in Pyrus regulates stigmatic ROS production to control pollen tube growth

文献类型： 外文期刊

作者： Zhang, Hao 1 ; Liu, Xueying 1 ; Tang, Chao 1 ; Qi, Kaijie 1 ; Xie, Zhihua 1 ; Wang, Peng 1 ; Ge, Tong 1 ; Xie, Zhu 1 ; Zhang, Mingliang 1 ; Wang, Yicheng 1 ; Gao, Zhenghui 2 ; Bao, Jianping 3 ; Zhang, Ningyi 1 ; de Graaf, Barend H. J. 4 ; Zhang, Shaoling 1 ; Wu, Juyou 1 ;

作者机构： 1.Nanjing Agr Univ, Sanya Inst, Natl Key Lab Crop Genet & Germplasm Enhancement &, Nanjing 210095, Jiangsu, Peoples R China

2.Anhui Acad Agr Sci, Inst Hort Res, Key Lab Hort Crop Germplasm Innovat & Utilizat, Co Construct Minist & Prov, Hefei 230031, Peoples R China

3.Tarim Univ, Coll Hort & Forestry, Alar 843300, Peoples R China

4.Cardiff Univ, Sch Biosci, Cardiff CF10 3AX, Wales

5.Zhongshan Biol Breeding Lab, 50 Zhongling St, Nanjing 210014, Jiangsu, Peoples R China

6.Nanjing Agr Univ, Coll Hort, Jiangsu Key Lab Hort Crop Breeding, Nanjing 210095, Jiangsu, Peoples R China

期刊名称：PLANT PHYSIOLOGY （ 影响因子：6.9； 五年影响因子：7.7 ）

ISSN： 0032-0889

年卷期： 2025 年 198 卷 4 期

页码：

收录情况： SCI

摘要： Accumulation of reactive oxygen species (ROS) in the stigma is crucial for the sexual reproduction of flowering plants. However, the derivation of stigmatic production of ROS in pear is still largely unknown. Here, we identified a respiratory burst oxidase homolog (Rboh) protein in pear (Pyrus bretschneideri), PbRbohD1, which is required for ROS accumulation in the stigma. Knockdown of PbRbohD1 by the antisense oligodeoxynucleotide (as-ODN) technique resulted in significant reductions of stigma ROS levels. Interaction analysis indicated that the N-terminal region of PbRbohD1 interacted with the stigmatic Rho of plant (ROP) protein PbrROP2, and PbRbohD1 colocalized with PbrROP2 in the plasma membrane. PbrROP2 activated the PbRbohD1 protein, thereby stimulating the production of ROS. Knockdown of PbrROP2 in the stigma drastically reduced the ROS levels. Thus, during pear stigma development, PbrROP2 and PbRbohD1 work together to control the stigmatic ROS level. Reducing the stigmatic ROS level with a ROS scavenger, sodium salicylate (Na-SA), accelerated the growth of self-pollen tubes in the style and increased the fruit set rate. Our results uncover a signaling pathway that regulates the levels of stigmatic ROS, providing the physiological basis for improving pollination efficiency and fruit set rate. The Rho of plant protein PbrROP2-respiratory burst oxidase homolog protein PbRbohD1 module regulates the production of reactive oxygen species in pear stigmas to control pollen tube growth.

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