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Identification of differentially expressed genes in longissimus dorsi muscle between Wei and Yorkshire pigs using RNA sequencing

文献类型： 外文期刊

作者： Xu, Jingen 1 ; Wang, Chonglong 3 ; Jin, Erhui 1 ; Gu, Youfang 1 ; Li, Shenghe 1 ; Li, Qinggang 3 ;

作者机构： 1.Anhui Sci & Technol Univ, Coll Anim Sci, Fengyang 233100, Anhui, Peoples R China

2.Anhui Prov Key Lab Local Livestock & Poultry Gene, Hefei 230036, Anhui, Peoples R China

3.Anhui Acad Agr Sci, Inst Anim Husb & Vet Med, Hefei 230031, Anhui, Peoples R China

关键词： Transcriptome sequencing; Pig; Meat quality; Breed; Differentially expressed genes; Intramuscular fat

期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）

ISSN： 1976-9571

年卷期： 2018 年 40 卷 4 期

页码：

收录情况： SCI

摘要： Intramuscular fat (IMF) content is an important trait closely related to meat quality, which is highly variable among pig breeds from diverse genetic backgrounds. High-throughput sequencing has become a powerful technique for analyzing the whole transcription profiles of organisms. In order to elucidate the molecular mechanism underlying porcine meat quality, we adopted RNA sequencing to detect transcriptome in the longissimus dorsi muscle of Wei pigs (a Chinese indigenous breed) and Yorkshire pigs (a Western lean-type breed) with different IMF content. For the Wei and Yorkshire pig libraries, over 57 and 64 million clean reads were generated by transcriptome sequencing, respectively. A total of 717 differentially expressed genes (DEGs) were identified in our study (false discovery rate < 0.05 and fold change > 2), with 323 up-regulated and 394 down-regulated genes in Wei pigs compared with Yorkshire pigs. Gene Ontology analysis showed that DEGs significantly related to skeletal muscle cell differentiation, phospholipid catabolic process, and extracellular matrix structural constituent. Pathway analysis revealed that DEGs were involved in fatty acid metabolism, steroid biosynthesis, glycerophospholipid metabolism, and protein digestion and absorption. Quantitative real time PCR confirmed the differential expression of 11 selected DEGs in both pig breeds. The results provide useful information to investigate the transcriptional profiling in skeletal muscle of different pig breeds with divergent phenotypes, and several DEGs can be taken as functional candidate genes related to lipid metabolism (ACSL1, FABP3, UCP3 and PDK4) and skeletal muscle development (ASB2, MSTN, ANKRD1 and ANKRD2).

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