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A novel switchable fluorescent sensor for facile and highly sensitive detection of alkaline phosphatase activity in a water environment with gold/silver nanoclusters

文献类型： 外文期刊

作者： Wang, Xiaoyan 1 ; Liu, Zhenjiang 1 ; Zhao, Wanying 1 ; Sun, Jianfan 1 ; Qian, Bin 1 ; Wang, Xinwei 1 ; Zeng, Huawei 2 ; D 1 ;

作者机构： 1.Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Jiangsu, Peoples R China

2.Huaibei Normal Univ, Coll Life Sci, Huaibei 235099, Anhui, Peoples R China

3.Anhui Acad Agr Sci, Minist Agr, Key Lab Agroprod Safety Risk Evaluat Hefei, Inst Plant Protect & Agroprod Safety, Hefei 230031, Anhui, Peoples R China

关键词： Au; Ag nanoclusters stabilized with bovine serum albumin; Alkaline phosphate activity; Fluorescence quenching; recovery

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN： 1618-2642

年卷期： 2019 年 411 卷 5 期

页码：

收录情况： SCI

摘要： A novel fluorescent sensor based on bovine serum albumin stabilized gold/silver nanoclusters (BSA-Au/Ag NCs) was developed for sensitive and facile detection of alkaline phosphatase (ALP) activity. For this fluorescent sensor, ascorbic acid 2-phosphate (AAP) was decomposed into ascorbic acid (AA) and phosphate by catalysis with ALP. The initial red fluorescence of the BSA-Au/Ag NCs was effectively quenched by KMnO4 and then the fluorescence was recovered by addition of AA. The mechanism of interaction between BSA-Au/Ag NCs and KMnO4 and AA was studied with use of the fluorescence lifetime and UV-vis absorption spectra. The results indicated that the oxidation/reduction modulated by KMnO4/AA led to surface structure destruction/restoration of the BSA-Au/Ag NCs, resulting in fluorescence quenching/recovery. The proposed fluorescence-based method based on a dark background was used to detect ALP and had excellent sensitivity, with a detection limit of 0.00076 U/L. Moreover, the method was applied to the determination of added analytes, with satisfactory recoveries (97.0-105.0 %). In a simulated eutrophic water body, this method successfully detected ALP in actual water samples and could monitor the dynamic changes of ALP activity through visual observation. More importantly, the proposed fluorescent sensor not only has the advantages of simple operation and high sensitivity but has also been successfully used on filter paper to establish a rapid and visual test paper for ALP.

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