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Cascade Amplifying Electrochemical Bioanalysis for Zearalenone Detection in Agricultural Products: Utilizing a Glucose-Fenton-HQ System on Bimetallic-ZIF@CNP Nanocomposites

文献类型： 外文期刊

作者： Liu, Guoxing 1 ; Liu, Zhaoying 1 ; Sun, Yumeng 1 ; Sun, Mingna 3 ; Duan, Jinsheng 3 ; Tian, Ye 1 ; Du, Daolin 4 ; Li, Ming 1 ;

作者机构： 1.Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Peoples R China

2.Chongqing Coll Mobile Commun, Sch Informat Secur, Chongqing 401420, Peoples R China

3.Anhui Acad Agr Sci, Minist Agr, Inst Plant Protect & Agroprod Safety, Key Lab Agroprod Safety Risk Evaluat, Hefei 230031, Peoples R China

4.Jiangsu Univ, Jingjiang Coll, Sch Environm & Safety Engn, Sch Emergency Management, Zhenjiang 212013, Peoples R China

5.Suzhou Univ Sci & Technol, Jiangsu Collaborat Innovat Ctr Technol & Mat Water, Sch Environm Sci & Engn, Suzhou 215009, Peoples R China

关键词： electrochemical bioanalysis; B-ZIF@CNP; glucose-Fenton-HQ system; cascade amplification; ultrasensitive detection

期刊名称：FOODS （ 影响因子：5.1； 五年影响因子：5.6 ）

ISSN：

年卷期： 2024 年 13 卷 19 期

页码：

收录情况： SCI

摘要： The integration of advanced materials and signaling cascade strategies is a promising and highly relevant topic for enhancing the performance of bioanalysis. In this study, a three-stage cascade amplification electrochemical bioanalysis (TCAE-bioanalysis) was developed and evaluated for the detection of zearalenone (ZEN). This method couples immunoreaction with a glucose-Fenton-hydroquinone (HQ) system on bimetallic-ZIF (B-ZIF)@CNP nanocomposites. The B-ZIF@CNP-modified gold electrode (AuE) was prepared, offering high conductivity and an excellent reaction interface. The immunoreaction introduced glucose oxidase (GOx) into the glucose-Fenton-HQ system, generating an abundant electron signal. The method achieved an ultrasensitive limit of detection (LOD) as low as 0.87 pg/mL, with an IC50 value of 30.8 pg/mL, representing a 229-fold enhancement in sensitivity compared to ELISA using the same monoclonal antibody (McAb). The specificity, reliability, and practicality of this approach were thoroughly demonstrated for agricultural product samples. Additionally, the TCAE-bioanalysis offers several advantages, including simplified preparation for advanced B-ZIF@CNP, a convenient detection system, and the use of common and environmentally friendly reagents. This study presents a comprehensive approach to improving electrochemical bioanalysis and may also expand the application of signaling cascades and environmentally friendly techniques in other biosensing or diagnostic contexts.

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