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Exo I-based cyclic digestion coupled with synergistic enhancement strategy for integrating dual-mode optical aptasensor platform

文献类型： 外文期刊

作者： Li, Ming 1 ; Teng, Weipeng 1 ; Lu, Wenying 1 ; Sun, Mingna 2 ; Duan, Jinsheng 2 ; Qiu, Xuchun 1 ;

作者机构： 1.Jiangsu Univ, Inst Environm Hlth & Ecol Secur, Sch Emergency Management, Sch Environm & Safety Engn, Zhenjiang 212013, Peoples R China

2.Anhui Acad Agr Sci, Minist Agr, Inst Plant Protect & Agroprod Safety, Key Lab Agroprod Safety Risk Evaluat, Hefei 230031, Peoples R China

3.Suzhou Univ Sci & Technol, Jiangsu Collaborat Innovat Ctr Technol & Mat Water, Sch Environm Sci & Engn, Suzhou 215009, Peoples R China

关键词： Dual-mode detection; Optical aptasensor; Exo I; Synergistic enhancement; Small molecule

期刊名称：TALANTA （ 影响因子：5.6； 五年影响因子：5.0 ）

ISSN： 0039-9140

年卷期： 2024 年 276 卷

页码：

收录情况： SCI

摘要： The improvement of dual-mode techniques was of particular interest to researchers, which might enhance the detection performance and applicability. Here, a dual-mode optical aptasensor (DO-aptasensor) platform based on exonuclease I (Exo I) cyclic digestion and synergistic enhancement strategy had proposed for zearalenone (ZEN). Following the preparation of dumbbell-shaped signal probe, the Exo I-based cyclic digestion amplification performed, and then the synergistic enhancement effect carried out to achieve the Poly-HRP-based colorimetry and FAM-SGI-based fluorescence. The efficient homogeneous system realized through the magnetic separation, while the signal interference further eliminated by the graphene oxide (GO). The LOD values were as low as 0.067 ng mL-1 for colorimetry mode and 0.009 ng mL-1 for fluorescence mode, which reduced 23-fold and 172fold than ELASA by same ZEN-Apt. This promising platform gave rise to a dual-mode optical readout, improved sensitivity and positively correlated detection. Meanwhile, the DO-aptasensor also exhibited the acceptable specificity, desirable reliability and excellent practicability. This novel avenue of aptasensor platform hold great potential for dual-mode optical monitoring of other targets, which can further expand the application scope of Exo I-based signal amplification and synergistic enhancement effect.

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