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Development of immunoassays based on specific monoclonal antibody for sensitive detection of azoxystrobin residues

文献类型： 外文期刊

作者： Salman, Hagar M. S. 1 ; You, Tianyang 1 ; Zhu, Jie 1 ; Kahiel, Mohamed 3 ; Wang, Minghua 1 ; Duan, Jinsheng 1 ; Hua, Xiude 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, State Key Lab Agr & Forestry Biosecur, Nanjing 210095, Peoples R China

2.Menoufia Univ, Dept Pesticides, Fac Agr, Shibin Al Kawm 32511, Mnf, Egypt

3.Menoufia Univ, Fac Agr, Dept Poultry & Fish Prod, Shibin Al Kawm 32511, Mnf, Egypt

4.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei 230031, Peoples R China

关键词： Azoxystrobin; Monoclonal antibody; Indirect competitive enzyme-linked immunosorbent assay; Immunochromatographic assay

期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）

ISSN： 0026-265X

年卷期： 2025 年 216 卷

页码：

收录情况： SCI

摘要： Azoxystrobin (AZX) is a widely used fungicide for the prevention and management of agricultural diseases, but its adverse effects on non-target organisms have raised concerns. In this study, monoclonal antibodies (mAbs) against AZX were developed to establish an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold immunochromatographic assay (GICA) for AZX detection. Eight hybridoma cell lines capable of stably secreting anti-AZX mAbs were prepared, among which the mAb 2A3C6 with the highest sensitivity was used in the immunoassays. The ic-ELISA showed a half-maximal inhibitory concentration (IC50) of 0.61 mu g L-1, and the GICA demonstrated a visual limit of detection (vLOD) of 0.6 mu g L-1. Both assays demonstrated high specificity, with no cross-reactivity observed for AZX analogues. Spiked recovery and blind sample detection confirmed the excellent sensitivity and reliability of these immunoassays for detecting AZX in environmental and agricultural samples.

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