您好，欢迎访问安徽省农业科学院 机构知识库！

Metabolomic Profiles of Bovine Mammary Epithelial Cells Stimulated by Lipopolysaccharide

文献类型： 外文期刊

作者： Huang, Yixin 1 ; Shen, Liuhong 1 ; Jiang, Jing 1 ; Xu, Qipin 1 ; Luo, Zhengzhong 1 ; Luo, Qiao 1 ; Yu, Shumin 1 ; Yao, Xu 1 ;

作者机构： 1.Sichuan Agr Univ, Coll Vet Med, Dept Clin Vet Med, Chengdu 611130, Sichuan, Peoples R China

2.Sichuan Prov Key Lab Anim Dis & Human Hlth, Chengdu 611130, Sichuan, Peoples R China

3.Univ Glasgow, Coll Med Vet & Life Sci, Inst Biodivers Anim Hlth & Comparat Med, Glasgow G61 1QH, Lanark, Scotland

4.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Hefei 230031, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2019 年 9 卷

页码：

收录情况： SCI

摘要： Bovine mammary epithelial cells (bMECs) are the main cells of the dairy cow mammary gland. In addition to their role in milk production, they are effector cells of mammary immunity. However, there is little information about changes in metabolites of bMECs when stimulated by lipopolysaccharide (LPS). This study describes a metabolomics analysis of the LPS-stimulated bMECs to provide a basis for the identification of potential diagnostic screening biomarkers and possible treatments for bovine mammary gland inflammation. In the present study, bMECs were challenged with 500 ng/mL LPS and samples were taken at 0 h, 12 h and 24 h post stimulation. Metabolic changes were investigated using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF MS) with univariate and multivariate statistical analyses. Clustering and metabolic pathway changes were established by MetaboAnalyst. Sixty-three differential metabolites were identified, including glycerophosphocholine, glycerol-3-phosphate, L-carnitine, L-aspartate, glutathione, prostaglandin G2, alpha-linolenic acid and linoleic acid. They were mainly involved in eight pathways, including D-glutamine and D-glutamic acid metabolism; linoleic acid metabolism; alpha-linolenic metabolism; and phospholipid metabolism. The results suggest that bMECs are able to regulate pro-inflammatory, anti-inflammatory, antioxidation and energy-producing related metabolites through lipid, antioxidation and energy metabolism in response to inflammatory stimuli.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台