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Comparative transcriptome analysis of differentially expressed genes related to the physiological changes of yellow-green leaf mutant of maize

文献类型： 外文期刊

作者： Li, Tingchun 1 ; Yang, Huaying 1 ; Lu, Yan 3 ; Dong, Qing 1 ; Liu, Guihu 1 ; Chen, Feng 2 ; Zhou, Yingbing 1 ;

作者机构： 1.Anhui Acad Agr Sci, Tobacco Res Inst, Hefei, Peoples R China

2.Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA

3.Western Michigan Univ, Dept Biol Sci, Kalamazoo, MI 49008 USA

关键词： Yellow-green leaf; Chlorophyll biosynthesis; Photosynthesis; Tricarboxylic acid cycle; Secondary metabolism; Transcriptome analysis

期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）

ISSN： 2167-8359

年卷期： 2021 年 9 卷

页码：

收录情况： SCI

摘要： Chlorophylls, green pigments in chloroplasts, are essential for photosynthesis. Reduction in chlorophyll content may result in retarded growth, dwarfism, and sterility. In this study, a yellow-green leaf mutant of maize, indicative of abnormity in chlorophyll content, was identified. The physiological parameters of this mutant were measured. Next, global gene expression of this mutant was determined using transcriptome analysis and compared to that of wild-type maize plants. The yellow-green leaf mutant of maize was found to contain lower contents of chlorophyll a, chlorophyll b and carotenoid compounds. It contained fewer active PSII centers and displayed lower values of original chlorophyll fluorescence parameters than the wild-type plants. The real-time fluorescence yield, the electron transport rate, and the net photosynthetic rate of the mutant plants showed reduction as well. In contrast, the maximum photochemical quantum yield of PSII of the mutant plants was similar to that of the wild-type plants. Comparative transcriptome analysis of the mutant plants and wild-type plants led to the identification of differentially expressed 1,122 genes, of which 536 genes were up-regulated and 586 genes down-regulated in the mutant. Five genes in the chlorophyll metabolism pathway, nine genes in the tricarboxylic acid cycle and seven genes related to the conversion of sucrose to starch displayed down-regulated expression. In contrast, genes encoding a photosystem II reaction center PsbP family protein and the PGR5-like protein 1A (PGRL1A) exhibited increased transcript abundance.

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