Identification and functional analysis of ZmDLS associated with the response to biotic stress in maize
文献类型： 外文期刊
作者： Wang, Yiting 1 ; Zou, Jie 1 ; Li, Jiali 1 ; Kong, Fanna 2 ; Xu, Lina 3 ; Xu, Dafeng 2 ; Li, Jiaxin 2 ; Yang, Huaying 2 ; Zhang, Lin 2 ; Li, Tingchun 2 ; Fan, Honghong 1 ;
作者机构： 1.Anhui Agr Univ, Sch Life Sci, Hefei, Peoples R China
2.Anhui Acad Agr Sci, Tobacco Res Inst, Hefei, Peoples R China
3.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei, Peoples R China
关键词： maize; terpene synthase; corn borer bite; Fusarium graminearum attack; defense response; transcription factor
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）
ISSN： 1664-462X
年卷期： 2023 年 14 卷
页码：
收录情况： SCI
摘要： Terpenes are the main class of secondary metabolites produced in response to pest and germ attacks. In maize (Zea mays L.), they are the essential components of the herbivore-induced plant volatile mixture, which functioned as a direct or indirect defense against pest and germ attacks. In this study, 43 maize terpene synthase gene (ZmTPS) family members were systematically identified and analyzed through the whole genomes of maize. Nine genes, including Zm00001d032230, Zm00001d045054, Zm00001d024486, Zm00001d004279, Zm00001d002351, Zm00001d002350, Zm00001d053916, Zm00001d015053, and Zm00001d015054, were isolated for their differential expression pattern in leaves after corn borer (Ostrinia nubilalis) bite. Additionally, six genes (Zm00001d045054, Zm00001d024486, Zm00001d002351, Zm00001d002350, Zm00001d015053, and Zm00001d015054) were significantly upregulated in response to corn borer bite. Among them, Zm00001d045054 was cloned. Heterologous expression and enzyme activity assays revealed that Zm00001d045054 functioned as d-limonene synthase. It was renamed ZmDLS. Further analysis demonstrated that its expression was upregulated in response to corn borer bites and Fusarium graminearum attacks. The mutant of ZmDLS downregulated the expressions of Zm00001d024486, Zm00001d002351, Zm00001d002350, Zm00001d015053, and Zm00001d015054. It was more attractive to corn borer bites and more susceptible to F. graminearum infection. The yeast one-hybrid assay and dual-luciferase assay showed that ZmMYB76 and ZmMYB101 could upregulate the expression of ZmDLS by binding to the promoter region. This study may provide a theoretical basis for the functional analysis and transcriptional regulation of terpene synthase genes in crops.
- 相关文献
作者其他论文 更多>>
-
In vitro activity of seven antifungal agents against Fusarium oxysporum and expression of related regulatory genes
作者：Xu, Dafeng;Li, Tingchun;Wang, Shiji;Kong, Fanna;Zhou, Benguo;Wang, Kun;Ruan, Banfeng;Wang, Jingjing;Dai, Jie;Liu, Yuguo
关键词：
-
Root exudates mediate tobacco microbial community remodeling and resistance to bacterial wilt disease
作者：Xia, Hao;Jiang, Chaoqiang;Yang, Huaying;Zu, Chaolong;Pan, Xiaolu;Shen, Jia;Riaz, Muhammad;Zhang, Guo;Wang, Xueying
关键词：Ralstonia solanacearum; phenolic acid; organic acid; soil microorganisms; tobacco variety
-
Risk Assessment and Fitness Cost of Tetraniliprole Resistance in Ostrinia furnacalis (Lepidoptera: Crambidae)
作者：Xu, Tingting;Hu, Fei;Yue, Ran;Hu, Benjin;Bi, Sijia;Tong, Youmin;Xu, Lina;Xu, Tingting;Hu, Fei;Hu, Benjin;Bi, Sijia;Tong, Youmin;Xu, Lina;Yue, Ran
关键词：
Ostrinia furnacalis ; tetraniliprole; risk assessment; fitness cost -
The Potential of Using Different Legume Species in a Push-Pull Approach to Manage Spodoptera frugiperda in Maize in China
作者：Bi, Sijia;Wang, Yinhao;Xu, Tingting;Hu, Benjin;Hu, Fei;Xu, Lina;Wang, Yinhao;Wang, Zhenying;Jatoi, Wajid Ali
关键词：cowpea; fall armyworm; mung bean; push-pull strategy; repellent and attractant plants
-
Transcriptome Analysis Reveals Genes and Pathways Associated with Drought Tolerance of Early Stages in Sweet Potato (Ipomoea batatas (L.) Lam.)
作者：Cheng, Peng;Kong, Fanna;Han, Yang;Liu, Xiaoping;Xia, Jiaping
关键词：sweet potato; drought stress; transcriptome; RNA-seq; root; leaf
-
Influence of climatic variables on maize grain yield and its components by adjusting the sowing date
作者：Wu, Wenming;Bi, Jianjian;Zhang, Lin;Xu, Dafeng;Peng, Chen;Wang, Shiji;Yue, Wei;Bi, Jianjian;Chen, Xiang
关键词：maize yield; sowing date; kernel number per ear; climatic variables; high temperature
-
Microbial regulatory mechanisms of disease-resistant tobacco varieties in the prevention and control of bacterial wilt disease
作者：Xia, Hao;Shen, Jia;Yang, Huaying;Dong, Qing;Zu, Chaolong;Yu, Fei;Yan, Yifeng;Li, Jiaxin;Jiang, Chaoqiang;Liu, Bo;Riaz, Muhammad
关键词：Bacterial wilt disease; Different variety; Rhizosphere microorganism; Soil metabolites; Physicochemical properties
