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Functional Analysis and Precise Location of m-1a in Rice

文献类型： 外文期刊

作者： Dong, Qing 1 ; Shen, Jia 1 ; Wang, Fang 1 ; Qi, Yaocheng 1 ; Jiang, Chaoqiang 1 ; Zu, Chaolong 1 ; Li, Tingchun 1 ;

作者机构： 1.Anhui Acad Agr Sci, Hefei 230031, Peoples R China

关键词： T-DNA; insertion mutant; chalkiness; amylose; starch synthesis genes

期刊名称：AGRONOMY-BASEL （ 影响因子：3.949； 五年影响因子：4.117 ）

ISSN：

年卷期： 2022 年 12 卷 7 期

页码：

收录情况： SCI

摘要： The T-DNA insertion technique is widely used in molecular breeding for its stable inheritance and low copy number in the plant genome. In our experiment, a transfer DNA (T-DNA) insertion grain of m-1a in rice was identified. Phenotypic analysis revealed that the grains appeared chalky and became extensive. The epidermis was shrinking. Meanwhile, the amylose contents of the seeds decreased significantly, and the expression of the most starch synthesis genes was obviously downregulated. Using the whole-genome sequencing and chromosome step method, the insertion position was uncovered and only located in Chr11 between 23266185 and 23266186 bp. These results may provide material for opening up new T-DNA insertion position points and a theoretical basis for rice molecular breeding.

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