A ribonuclease T2 protein FocRnt2 contributes to the virulence of Fusarium oxysporum f. sp. cubense tropical race 4
文献类型： 外文期刊
作者： He, Yanqiu 1 ; Li, Pengfei 1 ; Zhou, Xiaoshu 1 ; Ali, Shaukat 1 ; Zhu, Jie 1 ; Ma, Yini 1 ; Li, Jieling 1 ; Zhang, Nan 1 ; Li, Huaping 1 ; Li, Yunfeng 1 ; Nie, Yanfang 1 ;
作者机构： 1.South China Agr Univ, Coll Plant Protect, Guangdong Prov Key Lab Microbial Signals & Dis Con, Guangzhou, Peoples R China
2.Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei, Peoples R China
3.South China Agr Univ, Coll Mat & Energy, Guangzhou, Peoples R China
关键词： effector; Fusarium oxysporum f. sp. cubense; ribonucleases T2; transient expression; virulence
期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：4.8； 五年影响因子：6.0 ）
ISSN： 1464-6722
年卷期： 2024 年 25 卷 8 期
页码：
收录情况： SCI
摘要： Banana Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), is a major disease of banana plants worldwide. Effector proteins play critical roles in banana-Foc TR4 interaction. Our previous studies highlighted a ribonuclease protein belonging to the T2 family (named as FocRnt2) in the Foc TR4 secretome, which was predicted to be an effector. However, its biological function in Foc TR4 infection is still unclear. Herein, we observed significant expression of FocRnt2 during the early stage of fungal infection in planta. A yeast signal sequence trap assay showed that FocRnt2 contained a functional signal peptide for secretion. FocRnt2 possessed ribonuclease activity that could degrade the banana total RNA in vitro. Subcellular localization showed that FocRnt2 was localized in the nucleus and cytoplasm of Nicotiana benthamiana leaves. Transient expression of FocRnt2 suppressed the expression of salicylic acid- and jasmonic acid-signalling marker genes, reactive oxygen species accumulation, and BAX-mediated cell death in N. benthamiana. FocRnt2 deletion limited fungal penetration, reduced fusaric acid biosynthesis in Foc TR4, and attenuated fungal virulence against banana plants, but had little effect on Foc TR4 growth and sensitivity to various stresses. Furthermore, FocRnt2 deletion mutants induced higher expression of the defence-related genes in banana plants. These results suggest that FocRnt2 plays an important role in full virulence of Foc TR4, further improving our understanding of effector-mediated Foc TR4 pathogenesis.
- 相关文献
作者其他论文 更多>>
-
Effects on stone cell development and lignin deposition in pears by different pollinators
作者：Yan, Chongchong;Qi, Yongjie;Yan, Chongchong;Qi, Yongjie;Zhang, Nan;Xu, Chao;Jin, Qing;Cai, Yongping;Zhang, Nan
关键词：'Dangshan Su' pear; pollination; stone cell clusters; microscopy; ultramicroscopy
-
Screening of Candidate Effectors from Magnaporthe oryzae by In Vitro Secretomic Analysis
作者：Li, Guanjun;Shi, Qingchuan;Zhu, Jie;Zhong, Mingluan;Tong, Lingjie;Li, Huaping;Nie, Yanfang;Li, Yunfeng;He, Yanqiu;Nie, Yanfang
关键词：Magnaporthe oryzae; secretome; shotgun; effectors; bioinformatics
-
Full-length transcriptome sequencing reveals the molecular mechanism of potato seedlings responding to low-temperature
作者：Yan, Chongchong;Wang, Qianqian;Fu, Yuying;Zhao, Hongyuan;Wang, Jiajia;Wu, Gang;Liao, Huajun;Zhang, Nan;Wang, Feng;Li, Xueyan
关键词：potato; Second-generation sequencing technologies; Third-generation sequencing technologies; Full-length transcriptomes; Low-temperature stress
-
Development and performance evaluation of a wet-resistant strip-till seeder for sowing wheat following rice
作者：Li, Chaosu;Tang, Yonglu;Wu, Xiaoli;Liu, Miao;Li, Ming;Xiong, Tao;McHugh, Allen D.;Ling, Dong;Tang, Qing;Liao, Min;Du, Shizhou;Zhu, Jie;Huang, Yan
关键词：Strip-till seeder; Wheat following rice; Residue retention; Seedling establishment
-
The Effect of Low Temperature Stress on the Leaves and MicroRNA Expression of Potato Seedlings
作者：Yan, Chongchong;Wang, Qianqian;Fu, Yuying;Liao, Huajun;Zhang, Nan;Wang, Feng;Su, Yanbin;Zhou, Lin;Xue, Bingjie
关键词：abscisic acid; low temperature stress; miRNA; potato; qRT-PCR
-
High-Throughput MicroRNA and mRNA Sequencing Reveals that MicroRNAs May Be Involved in Pectinesterase-Mediated Cold Resistance in Potato
作者：Yan, Chongchong;Wang, Qianqian;Wang, Jiajia;Ren, Xuexiang;Liao, Huajun;Zhang, Nan;Xue, Bingjie;Pu, Xiaojing;Xu, Zhihong
关键词：Potato; microRNA; RNA-Seq; low-temperature stress; pectinesterase
-
The Effect of Different Pollination on the Expression of Dangshan Su Pear MicroRNA
作者：Cheng, Xi;Yan, Chongchong;Zhang, Jinyun;Ma, Chenhui;Li, Shumei;Jin, Qing;Zhang, Nan;Cao, Yunpeng;Lin, Yi;Cai, Yongping;Yan, Chongchong;Zhang, Jinyun
关键词：
