Comparison Between Environmental DNA Metabarcoding and Traditional Survey Method to Identify Community Composition and Assembly of Stream Fish
文献类型： 外文期刊
作者： Liu, Ziyu 1 ; Hu, Cong 1 ; You, Wenhui 1 ; Li, Shuxin 1 ; Wu, Yongsheng 1 ; Liang, Yangyang 2 ; Chu, Ling 1 ; Yan, Yunzhi 1 ; Zhang, Chen 1 ;
作者机构： 1.Anhui Normal Univ, Sch Ecol & Environm, Wuhu, Peoples R China
2.Anhui Acad Agr Sci, Anhui Prov Key Lab Aquaculture & Stock Enhancement, Fishery Inst, Hefei, Peoples R China
3.Anhui Normal Univ, Prov Key Lab Biot Environm & Ecol Safety Anhui, Wuhu, Anhui, Peoples R China
4.Anhui Normal Univ, Collaborat Innovat Ctr Recovery & Reconstruct Degr, Wuhu, Peoples R China
5.Anhui Normal Univ, Minist Educ, Wuhu, Peoples R China
关键词： community assembly; functional diversity; null model; subtropical river
期刊名称：ECOLOGY AND EVOLUTION （ 影响因子：2.3； 五年影响因子：2.8 ）
ISSN： 2045-7758
年卷期： 2024 年 14 卷 11 期
页码：
收录情况： SCI
摘要： Environmental DNA (eDNA) metabarcoding has been widely used in freshwater systems, contributing to the advancements in the monitoring of fish diversity and community species composition. Nevertheless, the accuracy and reliability of eDNA metabarcoding in assessing functional structures and revealing the mechanisms underlying fish community assembly remain unclear. In this study, we combined a traditional survey method (electrofishing) and eDNA metabarcoding to conduct fish stock monitoring in the upper reaches of the Huishui stream. We assessed taxonomic and functional structures, as well as community assembly mechanisms, during dry and wet seasons. The results revealed that, compared with electrofishing surveys, eDNA metabarcoding detected a greater number of species and higher functional richness in both seasons. Despite significant differences in fish taxonomic composition between the seasons, both eDNA and traditional methods indicated that environmental filtering dominated the process of fish community assembly in both dry and wet seasons. We showed that eDNA metabarcoding is comparable to the electrofishing method in monitoring the community composition of stream fish and can accurately and reliably determine fish community assembly mechanisms. Combining functional traits and eDNA is a robust approach for monitoring stream fish community compared to taxonomic uncertainty.
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