文献类型： 外文期刊

作者： Xie, CX ¹ ; Xu, A ² ; Wu, LJ ² ; Yao, JM ² ; Yang, JB ² ; Yu, ZL ² ;

作者机构： 1.Chinese Acad Sci, Inst Plasma Phys, Key Lab Ion Beam Bioengn, Hefei, Anhui, Peoples R China

2.Chinese Acad Sci, Inst Plasma Phys, Key Lab Ion Beam Bioengn, Hefei, Anhui, Peoples R China; Anhui Acad Agr Sci, Inst Atom Energy, Hefei, Anhui, Peoples R China

关键词： base substitution;low-energy nitrogen ion beam implantation;Co-60-gamma rays;Escherichia coli

期刊名称：GENETICS AND MOLECULAR BIOLOGY （ 影响因子：1.771； 五年影响因子：2.584 ）

ISSN： 1415-4757

年卷期： 2004 年 27 卷 2 期

页码：

收录情况： SCI

摘要： To identify the specificity of base substitutions, a novel experimental system was established based on rifampicin-resistant (Rif') mutant screening and sequencing of the defined region of the rpoB gene in E coli. We focused on comparing mutational spectra of base substitutions induced by either low energy nitrogen ion beam implantation or Co-60-gamma rays. The most significant difference in the frequency of specific kinds of mutations induced by low energy nitrogen ion beam was that CG --> TA transitions were significantly increased from 32 to 46, AT --> TA transversions were doubled from 7 to 15 in 50 mutants, respectively. The preferential base substitutions induced by nitrogen ion beam implantation were CG --> TA transitions, AT --> GC transitions, AT --> TA transversions, which account for 92.13% (82/89) of the total. The mutations induced by Co-60-gamma rays were preferentially GC --> AT and AT --> GC transitions, which totaled 84.31% (43/51).