文献类型： 外文期刊

作者： Xiang, TH ¹ ; Yang, JB ² ; Yang, QJ ² ; Zhu, QS ² ; Li, L ² ; Ni, DH ² ; Wang, XF ² ; Huang, DN ² ;

作者机构： 1.Natl Agr Minist, Key Lab Rice Genet & Breeding, Rice Res Inst, Anhui Acad Agr Sci, Hefei 230031, Peoples R China

2.Natl Agr Minist, Key Lab Rice Genet & Breeding, Rice Res Inst, Anhui Acad Agr Sci, Hefei 230031, Peoples R China; Zhejiang Univ, Life Coll, Hangzhou 310012, Peoples R China; Anhui Acad Agr Sci, Nat Nourishing Food Engn Inst, Hefei 230031, Peoples R China; China Natl Rice Res Inst, Hangzhou 310006, Peoples R China

关键词： Co-60 gamma-ray irradiation;rice (oryza sative);genome DNA;mutagenesis;DNA fragment deletion

期刊名称：PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS （ 影响因子：0.351； 五年影响因子：0.272 ）

ISSN： 1000-3282

年卷期： 2002 年 29 卷 5 期

页码：

收录情况： SCI

摘要： A total of 360 arbitrary 10-mer oligonucleotide primers were screened using RAPD (random amplified polymorphic DNA) technique on the genome DNA of Norm 8 and its Co-60 gamma-ray irradiation mutant Norin 8m. One primer OPG18 produced co-dominant polymorphic markers OPG18/972 and OPG18/943 from Norin 8 and Norm 8m. Based on the clones and sequences of the OPG18/972 and OPG18/943, it shows that a 29 bp DNA fragment in the genome DNA of Norin 8m is deleted. The results directly confirmed the fact that Co-60 gamma-ray irradiation can induced a bigger DNA fragment deletion of plant genome DNA.