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Single Nucleotide Polymorphism Scanning and Expression of the Pig PPARGC1A Gene in Different Breeds

文献类型： 外文期刊

作者： Li, Qinggang 1 ; Wang, Zhixiu 1 ; Zhang, Bo 1 ; Lu, Yunfeng 2 ; Yang, Yuzeng 1 ; Ban, Dongmei 1 ; Wu, Changxin 1 ; Zhang, 1 ;

作者机构： 1.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Hefei 230031, Anhui, Peoples R China

2.Nanyang Normal Univ, Sch Life Sci & Technol, Nanyang 473061, Henan Province, Peoples R China

关键词： Pig;Lipids;PPARGC1A;Gene expression;SNPs

期刊名称：LIPIDS （ 影响因子：1.88； 五年影响因子：2.417 ）

ISSN： 0024-4201

年卷期： 2014 年 49 卷 10 期

页码：

收录情况： SCI

摘要： Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a candidate gene for lean meat production because it plays a key role in lipid metabolism. In this study, SNPs within the porcine PPARGC1A gene were investigated using PCR-sequencing and PCR-RFLP. Quantitative real-time PCR and Western blot were then used to analyze mRNA and protein expression in longissimus dorsi muscle (LM), liver, and backfat tissues of Dianna small-ear pigs (DSP, n = 6), Tibetan pigs (TP, n = 6), and large white pigs (LW, n = 6). Five novel SNPs (g.-1269A > G in the 5'-upstream regulatory region; g.190C > T, g.218C > A and g.234C > A in exon 8; and g.20C > T in intron 10) and three previously identified SNPs (g.417A > T in exon 8; g.56C > A in exon 9; and g.34G > A in intron 9) were found. Of these, only two, g.-1269A > G and g.234C > A, had three different genotypes in the three breeds (DSP, n = 63; TP, n = 51; and LW, n = 52). Expression was highest in LM, modest in the liver, and minimal in backfat. In LM tissue, LW had higher mRNA and protein levels than DSP and TP (P < 0.05), and there was a negative correlation between gene expression and intramuscular fat (IMF) content. LW had numerically higher expression in liver and backfat tissues than DSP and TP, and the differences in protein levels were significant (P < 0.05 in liver, P < 0.01 in backfat). In conclusion, PPARGC1A may play a key role in down-regulating lipid deposition, and the SNPs with differential genotype distribution among the three pig breeds may be related to gene expression and fat deposition.

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