您好，欢迎访问安徽省农业科学院 机构知识库！

Low-Temperature-Induced Expression of Rice Ureidoglycolate Amidohydrolase is Mediated by a C-Repeat/Dehydration-Responsive Element that Specifically Interacts with Rice C-Repeat-Binding Factor 3

文献类型： 外文期刊

作者： Li, Juan 1 ; Qin, Rui-Ying 1 ; Li, Hao 1 ; Xu, Rong-Fang 1 ; Yang, Ya-Chun 1 ; Ni, Da-Hu 1 ; Ma, Hui 1 ; Li, Li 1 ; Wei, Pen 1 ;

作者机构： 1.Anhui Acad Agr Sci, Rice Res Inst, Key Lab Rice Genet Breeding Anhui Prov, Hefei, Peoples R China

关键词： CRT/DRE element;low temperature stress;OsCBF3;transcriptional regulation;ureidoglycolate amidohydrolase

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2015 年 6 卷

页码：

收录情况： SCI

摘要： Nitrogen recycling and redistribution are important for the environmental stress response of plants. In non-nitrogen-fixing plants, ureide metabolism is crucial to nitrogen recycling from organic sources. Various studies have suggested that the rate-limiting components of ureide metabolism respond to environmental stresses. However, the underlying regulation mechanism is not well understood. In this report, rice uradoglycolate amidohydrolase (OsUAH), which is a recently identified enzyme catalyzing the final step of ureide degradation, was identified as low-temperature- (LT) but not abscisic acid- (ABA) regulated. To elucidate the LT regulatory mechanism at the transcriptional level, we isolated and characterized the promoter region of OsUAH (PosuAH). Series deletions revealed that a minimal region between 522 and 420 relative to the transcriptional start site was sufficient for the cold induction of PosuAH. Detailed analyses of this 103 bp fragment indicated that a C-repeat/dehydration-responsive (CRT/DRE) element localized at position 434 was essential for LT-responsive expression. A rice C-repeat-binding factors/DRE-binding proteins 1 (CBE5/DREB1s) subfamily member, OsCBF3, was screened to specifically bind to the CRT/DRE element in the minimal region both in yeast one hybrid assays and in in vitro gel shift analysis. Moreover, the promoter could be exclusively trans-activated by the interaction between the CRT/DRE element and OsCBF3 in vivo. These findings may help to elucidate the regulation mechanism of stress-responsive ureide metabolism genes and provide an example of the member specific manipulation of the CBF/DREB1 subfamily.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台