文献类型: 外文期刊
作者: Li, Guan 1 ; Liu, Rong 1 ; Xu, Rongfang 2 ; Varshney, Rajeev K. 3 ; Ding, Hanfeng 4 ; Li, Mengwei 1 ; Yan, Xin 1 ; Huang, Shuxian 1 ; Li, Juan 2 ; Wang, Dong 1 ; Ji, Yishan 1 ; Wang, Chenyu 1 ; He, Junguang 5 ; Luo, Yingfeng 6 ; Gao, Shenghan 6 ; Wei, Pengcheng 2 ; Zong, Xuxiao 1 ; Yang, Tao 1 ;
作者机构: 1.Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Inst Crop Sci, Beijing 100081, Peoples R China
2.Anhui Acad Agr Sci, Rice Res Inst, Key Lab Rice Genet Breeding Anhui Prov, Hefei 230031, Anhui, Peoples R China
3.Murdoch Univ, Food Futures Inst, State Agr Biotechnol Ctr, Ctr Crop & Food Innovat, Murdoch, WA 6150, Australia
4.Shandong Acad Agr Sci, Inst Crop Germplasm Resources, Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan 250100, Shandong, Peoples R China
5.Zhejiang Xinnan Chem Ind Grp Co Ltd, Hangzhou 311300, Zhejiang, Peoples R China
6.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
关键词: Agrobacterium-mediated transformation; CRISPR/Cas9; Pea; Genome editing
期刊名称:CROP JOURNAL ( 影响因子:6.6; 五年影响因子:6.5 )
ISSN: 2095-5421
年卷期: 2023 年 11 卷 1 期
页码:
收录情况: SCI
摘要: Pea (Pisum sativum L.) is an annual cool-season legume crop. Owing to its role in sustainable agriculture as both a rotation and a cash crop, its global market is expanding and increased production is urgently needed. For both technical and regulatory reasons, neither conventional nor transgenic breeding tech-niques can keep pace with the demand for increased production. In answer to this challenge, CRISPR/ Cas9 genome editing technology has been gaining traction in plant biology and crop breeding in recent years. However, there are currently no reports of the successful application of the CRISPR/Cas9 genome editing technology in pea. We developed a transient transformation system of hairy roots, mediated by Agrobacterium rhizogenes strain K599, to validate the efficiency of a CRISPR/Cas9 system. Further opti-mization resulted in an efficient vector, PsU6.3-tRNA-PsPDS3-en35S-PsCas9. We used this optimized CRISPR/Cas9 system to edit the pea phytoene desaturase (PsPDS) gene, causing albinism, by Agrobacterium-mediated genetic transformation. This is the first report of successful generation of gene-edited pea plants by this route. (c) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
- 相关文献
作者其他论文 更多>>
-
Conditional knockdown of OsMLH1 to improve plant prime editing systems without disturbing fertility in rice
作者:Liu, Xiaoshuang;Zhang, Yiru;Jiang, Yingli;Xiao, Zhi;Wei, Pengcheng;Gu, Dongfang;Xu, Rongfang;Qin, Ruiying;Li, Juan;Wei, Pengcheng
关键词:Prime editing; DNA mismatch repair; Rice; Fertility; Cre recombinase
-
Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding
作者:Li, Yizhen;Liang, Jing;Deng, Bufang;Jiang, Yingli;Zhu, Jingyan;Chen, Like;Li, Min;Li, Juan
关键词:base editing; CRISPR; Cas9; plant; genome editing; crop improvement
-
Physiological and metabolic responses to nitrogen availability of rice (Oryza sativa L.) cultivars with differ in nitrogen efficient
作者:Ruan, Xinmin;Dong, Zhaorong;Ruan, Xinmin;Du, Hongyang;Zhan, Xinchun;Cong, Xihan;Shi, Fuzhi;Luo, Zhixiang;Li, Juan
关键词:Rice; Nitrogen-use efficiency (NUE); Metabolome; Carbon metabolism; Tricarboxylic acid (TCA)
-
An epi-allele of SMS causes Sanming dominant genic male sterility in rice
作者:Yang, Yachun;Li, Hao;Xu, Zuntao;Tai, Dewei;Ni, Dahu;Wei, Pengcheng;Yi, Chengxin;Yang, Jianbo;Yang, Yachun;Zhang, Cheng;Li, Hao;Xu, Zuntao;Tai, Dewei;Ding, Yong;Zhang, Cheng;Yang, Zeyuan;Xu, Zuntao;Ding, Yong
关键词:male sterility; rice; epi-allele; Sanming; DNA methylation
-
Natural variation in Tiller Number 1 affects its interaction with TIF1 to regulate tillering in rice
作者:Zhang, Quan;Xie, Jianyin;Zhu, Xiaoyang;Ma, Xiaoqian;Yang, Tao;Khan, Najeeb Ullah;Zhang, Shuyang;Liu, Miaosong;Li, Lin;Li, Jinjie;Li, Zichao;Zhang, Hongliang;Zhang, Zhanying;Liang, Yuntao;Pan, Yinghua;Li, Danting;Li, Zichao;Zhang, Hongliang;Zhang, Hongliang
关键词:rice; tiller number; BAH domain; GWAS; geographical distribution
-
Development of plant cytosine base editors with the Cas12a system
作者:Wang, Huanhuan;Liang, Jing;Chen, Like;Deng, Bufang;Liu, Xiaoshuang;Zhu, Yitong;Zhao, Liangxia;Chen, Yanjun;Jiang, Yingli;Wei, Pengcheng;Gu, Dongfang;Jin, Shan;Xu, Rongfang;Qin, Ruiying;Kou, Dourong;Li, Juan
关键词:Cas12a; Base editing; Rice; ABE; CBE
-
Transcriptome analysis of maize pollen grains under drought stress during flowering
作者:Zhang, Yinping;Soualihou, Soualiou;Xu, Yonghan;Li, Jincai;Song, Youhong;Zhang, Yinping;Soualihou, Soualiou;Li, Juan;Rose, Ray J.;Ruan, Yong-Ling;Song, Youhong
关键词:anthesis; differentially expressed genes; drought stress; pollen development; pollen vitality; reporductive tissue; Zea mays L