Weighted gene co-expression network analysis reveals key module and hub genes associated with the anthocyanin biosynthesis in maize pericarp
文献类型： 外文期刊
作者： Li, Tingchun 1 ; Wang, Yiting 2 ; Dong, Qing 1 ; Wang, Fang 1 ; Kong, Fanna 1 ; Liu, Guihu 1 ; Lei, Yanli 1 ; Yang, Huaying 1 ; Zhou, Yingbing 1 ; Li, Cheng 1 ;
作者机构： 1.Anhui Acad Agr Sci, Tobacco Res Inst, Hefei, Peoples R China
2.Anhui Agr Univ, Sch Life Sci, Hefei, Peoples R China
关键词： anthocyanins; pericarp; comparative dynamic transcriptome analysis; weighted gene co-expression network analysis; maize
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： Anthocyanins are the visual pigments that present most of the colors in plants. Its biosynthesis requires the coordinated expression of structural genes and regulatory genes. Pericarps are the rich sources of anthocyanins in maize seeds. In the experiment, the transcriptomes of transparent and anthocyanins-enriched pericarps at 15, 20, and 25 DAP were obtained. The results output 110.007 million raw reads and 51407 genes' expression matrix. Using data filtration in R language, 2057 genes were eventually identified for weighted gene co-expression network analysis. The results showed that 2057 genes were classified into ten modules. The cyan module containing 183 genes was confirmed to be the key module with the highest correlation value of 0.98 to the anthocyanins trait. Among 183 genes, seven structural genes were mapped the flavonoid biosynthesis pathway, and a transcription factor Lc gene was annotated as an anthocyanin regulatory gene. Cluster heatmap and gene network analysis further demonstrated that Naringenin, 2-oxoglutarate 3-dioxygenase (Zm00001d001960), Dihydroflavonol 4-reductase (Zm00001d044122), Leucoanthocyanidin dioxygenase (Zm00001d014914), anthocyanin regulatory Lc gene (Zm00001d026147), and Chalcone synthase C2 (Zm00001d052673) participated in the anthocyanins biosynthesis. And the transcription factor anthocyanin regulatory Lc gene Zm00001d026147 may act on the genes Chalcone synthase C2 (Zm00001d052673) and Dihydroflavonol 4-reductase (Zm00001d044122). The yeast one-hybrid assays confirmed that the Lc protein could combine with the promoter region of C2 and directly regulate the anthocyanin biosynthesis in the pericarp. These results may provide a new sight to uncover the module and hub genes related to anthocyanins biosynthesis in plants.
- 相关文献
作者其他论文 更多>>
-
Transcriptome Analysis Reveals Genes and Pathways Associated with Drought Tolerance of Early Stages in Sweet Potato (Ipomoea batatas (L.) Lam.)
作者：Cheng, Peng;Kong, Fanna;Han, Yang;Liu, Xiaoping;Xia, Jiaping
关键词：sweet potato; drought stress; transcriptome; RNA-seq; root; leaf
-
Microbial regulatory mechanisms of disease-resistant tobacco varieties in the prevention and control of bacterial wilt disease
作者：Xia, Hao;Shen, Jia;Yang, Huaying;Dong, Qing;Zu, Chaolong;Yu, Fei;Yan, Yifeng;Li, Jiaxin;Jiang, Chaoqiang;Liu, Bo;Riaz, Muhammad
关键词：Bacterial wilt disease; Different variety; Rhizosphere microorganism; Soil metabolites; Physicochemical properties
-
Identification and functional analysis of ZmDLS associated with the response to biotic stress in maize
作者：Wang, Yiting;Zou, Jie;Li, Jiali;Fan, Honghong;Kong, Fanna;Xu, Dafeng;Li, Jiaxin;Yang, Huaying;Zhang, Lin;Li, Tingchun;Xu, Lina
关键词：maize; terpene synthase; corn borer bite; Fusarium graminearum attack; defense response; transcription factor
-
The Changes in Rhizosphere Metabolome and Microbiota Are the Main Direct Obstacles to Continuous Cropping in Tobacco (Nicotiana tabacum L.)
作者：Yu, Fei;Yan, Yifeng;Dong, Qing;Jiang, Chaoqiang;Zu, Chaolong;Shen, Jia
关键词：continuous cropping obstacles; ecological cluster; metabolome; microbiota
-
Identification and complete genome sequence of iris potyvirus A, which causes dwarfing and foliar chlorosis with mosaic or mottle disease symptoms on lily (Lilium lancifolium Thunb.) in China
作者：Wang, Fang;Yan, Dankan;Han, Kelei;Ma, Chao;Chen, Ying;Gao, Zhengliang;Bao, Xianxun;Li, Cheng
关键词：
-
Complete genome sequence of triticum yellow stripe virus, a new polerovirus infecting wheat (triticum aestivum) in China
作者：Yan, Dankan;Han, Kelei;Chen, Ying;Ma, Chao;Hu, Shuzhen;Zhao, Wei;Wang, Fang
关键词：
-
Dynamic Transcriptome-Based Weighted Gene Co-expression Network Analysis Reveals Key Modules and Hub Genes Associated With the Structure and Nutrient Formation of Endosperm for Wax Corn
作者：Li, Tingchun;Kong, Fanna;Dong, Qing;Xu, Dafeng;Liu, Guihu;Lei, Yanli;Yang, Huaying;Zhou, Yingbing;Li, Cheng
关键词：waxy corn; floury endosperm; dynamic comparative transcriptome analysis; weighted gene co-expression network analysis; modules and hub genes
