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Porcine epidemic diarrhea virus infection blocks cell cycle and induces apoptosis in pig intestinal epithelial cells

文献类型： 外文期刊

作者： Shen, Xuehuai 1 ; Yin, Lei 1 ; Pan, Xiaocheng 1 ; Zhao, Ruihong 1 ; Zhang, Danjun 1 ;

作者机构： 1.Anhui Acad Agr Sci, Inst Anim Husb & Vet Sci, Hefei 230031, Anhui, Peoples R China

2.Livestock & Poultry Epidem Dis Res Ctr Anhui Prov, Hefei 230031, Anhui, Peoples R China

关键词： PEDV; IPEC-J2 cell; RNA-Seq; Cell cycle; Apoptosis

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

ISSN： 0882-4010

年卷期： 2020 年 147 卷

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV) is responsible for the acute infectious swine disease porcine epidemic diarrhea (PED). PED causes damage to the intestine, including villus atrophy and shedding, leading to serious economic losses to the pig industry worldwide. We carried out an in vitro study to investigate cell apoptosis and the cell cycle in a PEDV-infected host using transcriptomic shotgun sequencing (RNA-Seq) to study gene responses to PEDV infection. Results revealed that the PEDV infection reduced proliferation activity, blocked the cell cycle at S-phase and induced apoptosis in IPEC-J2 cells. The expression of gene levels related to ribosome proteins and oxidative phosphorylation were significantly up-regulated post-PEDV infection. Although the significantly down-regulated on PI3K/Akt signaling pathway post-PEDV infection, the regulator-related genes of mTOR signaling pathway exerted significantly up-regulated or down-regulated in IPEC-J2 cells. These results indicated that ribosome proteins and oxidative phosphorylation process were widely involved in the pathological changes and regulation of host cells caused by PEDV infection, and PI3K/AKT and mTOR signaling pathways played a vital role in antiviral regulation in IPEC-J2 cells. These data might provide new insights into the specific pathogenesis of PEDV infection and pave the way for the development of effective therapeutic strategies.

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