Beta-Sitosterol Enhances Classical Swine Fever Virus Infection: Insights from RNA-Seq Analysis
文献类型： 外文期刊
作者： Liu, Yayun 1 ; Yin, Dongdong 1 ; Wang, Jieru 1 ; Dai, Yin 1 ; Shen, Xuehuai 1 ; Yin, Lei 1 ; Zhou, Bin 3 ; Pan, Xiaocheng 1 ;
作者机构： 1.Anhui Acad Agr Sci, Inst Anim Husb & Vet Sci, Livestock & Poultry Epidem Dis Res Ctr Anhui Prov, Hefei 230031, Peoples R China
2.Anhui Prov Key Lab Livestock & Poultry Prod Safety, Hefei 230031, Peoples R China
3.Nanjing Agr Univ, Coll Vet Med, MOE Joint Int Res Lab Anim Hlth & Food Safety, Nanjing 210095, Peoples R China
关键词： beta-sitosterol (BS); classical swine fever virus (CSFV); lipid compounds; replication; differentially expressed genes (DEGs)
期刊名称：VIRUSES-BASEL （ 影响因子：3.5； 五年影响因子：3.7 ）
ISSN：
年卷期： 2025 年 17 卷 7 期
页码：
收录情况： SCI
摘要： Beta-sitosterol (BS), a naturally occurring phytosterol abundant in plants, has been reported to exhibit diverse biological activities, including immunomodulatory and antiviral effects. Classical swine fever virus (CSFV), a member of the Pestivirus genus, remains a persistent threat to the swine industry worldwide, causing considerable economic damage. Our research found that BS significantly enhances the replication of both the CSFV-Shimen strain and the attenuated C-strain vaccine virus in PK-15 cells. Additionally, transcriptomic profiling (RNA-Seq) identified 175 differentially expressed genes (DEGs) following BS exposure, comprising 53 upregulated and 122 downregulated genes. Further results demonstrated that treatment with beta-sitosterol suppressed I kappa B alpha expression, thereby activating the NF-kappa B pathway, and that knockdown of endogenous I kappa B alpha significantly promoted CSFV replication. These findings contribute to a deeper understanding of how BS influences the CSFV infection process, suggesting its role as a host lipid-associated factor facilitating viral propagation.
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