Identification and immunoregulatory role of cathepsin A in the red swamp crayfish, Procambarus clarkii
文献类型： 外文期刊
作者： Huang, Long 1 ; Dai, Li-Shang 1 ; Wu, Ben-Li 1 ; Zhang, Ye 1 ; Chen, Jing 1 ; He, Ji-Xiang 1 ;
作者机构： 1.Anhui Acad Agr Sci, Fishery Inst, Anhui Prov Key Lab Aquaculture & Stock Enhanceme, Hefei 230031, Peoples R China
2.Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Peoples R China
关键词： Crustaceans; RNA interference; Innate immunity; Microbial infection; Immune defence
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2020 年 153 卷
页码：
收录情况： SCI
摘要： Cathepsins are a group of lysosomal hydrolytic enzymes, broadly distributed in animals, and regulate various physiological processes. However, the immune functions of cathepsins are poorly understood in invertebrates. Therefore, to further provide information about the importance of cathepsins in the innate immune system of crustaceans, cathepsin A from Procambarus clarkii (Pc-cathepsin A) was characterized and its distribution in different tissues was determined. The immunological functions of the Pc-cathepsin A were also evaluated. The Pc-cathepsin A showed high sequence homology to cathepsins of other species, as it contained serine and histidine active sites. Quantitative RT-PCR analysis revealed that the expression of Pc-cathepsin A was highest in the gill, gut, and the hepatopancreas, with variable amounts in the muscle, stomach, heart, and hemocytes. The mRNA expression of Pc-cathepsin A was significantly increased in hepatopancreas challenged with lipopolysaccharide (LPS), peptidoglycan (PGN), and polycytidylic acid (poly I:C). The results of an in vivo analysis revealed that Pc-cathepsin A knockdown by double-stranded RNA in P. clarkii modulated the expression of immune-pathway associated genes in hepatopancreas. Collectively, these results suggest that Pc-cathepsin A modulates innate immune responses by affecting the expression of immune-pathway associated genes, thus revealing a regulatory link between Pc-cathepsin A and immune pathways in P. clarkii, and that Pc-cathepsin A plays an essential biological role in the immune defence against microbial pathogens. (C) 2020 Published by Elsevier B.V.
- 相关文献
作者其他论文 更多>>
-
Adaptation and feeding preference of Chinese soft-shelled turtle ( Pelodiscus sinensis) ) to different feeding levels in rice-turtle - turtle co-culture system
作者：Wu, Benli;Wu, Cangcang;Huang, Long;Chen, Jing;Chen, Xiajun;He, Jixiang
关键词：Feeding level; Rice-turtle co-culture; Growth performance; Behavioral and physiological responses; Gene expression
-
Effects of Functional Oligosaccharides Extracted from Straw on the Growth, Feeding, Physiology, Histology, Muscle Texture, and Gut Microbiota of Micropterus salmoides
作者：Wu, Benli;Huang, Long;Wu, Cangcang;Chen, Jing;Chen, Xiajun;Wang, Xiang;Cui, Kai;He, Jixiang;Liu, Zhigang
关键词：
-
Peroxiredoxin 1 of Procambarus clarkii govern immune responses during pathogen infection
作者：Huang, Long;Huang, Long;Liu, Yu;Zhang, Xinxin;Xu, Jie;Dai, Lishang;Huang, Lehao;Dai, Lu;Dai, Lu
关键词：Crustaceans; Peroxiredoxin; Antimicrobial immunity
-
A stable and major QTL region on chromosome 2 conditions pod shape in cultivated peanut (Arachis hyopgaea L.)
作者：Zhang, Sheng-Zhong;Hu, Xiao-Hui;Wang, Fei-fei F.;Yang, Wei-Qiang;Miao, Hua-Rong;Chen, Jing;Chu, Ye;Xu, Sheng;Wang, Song;Wu, Lan-Rong;Yu, Hao-Liang;Fu, Chun
关键词：peanut; pod shape; quantitative trait locus; additive effect; epistasis
-
Transcriptome analysis of differentially expressed genes in the red swamp crayfish Procambarus clarkii challenged with Aeromonas hydrophila
作者：Liu, Qiu-Ning;Huang, Long;Wang, Shu-Yu;He, Ji-Xiang;Liu, Qiu-Ning;Huang, Long;Wang, Shu-Yu;Li, Yue-Tian;Tang, Ying-Yu;Zhang, Dai-Zhen;Tang, Bo-Ping;He, Ji-Xiang;Liu, Qiu-Ning;Ding, Feng;Li, Yue-Tian;Li, Yue-Tian;Tang, Ying-Yu;Yang, Hui
关键词：Procambarus clarkii; Aeromonas hydrophila; Immune response; Transcriptome
-
Molecular characterization and functional analysis of the lysosomal cathepsin D-like gene in red swamp crayfish, Procambarus clarkii
作者：Huang, Long;Wu, Ben-li;He, Ji-Xiang;Zhang, Ye;Chen, Jing;Chen, Xia-jun
关键词：cathepsins; crayfish; immune genes; immune system; microbial infection
-
Nutritional evaluation of two strains of Chinese soft-shelled turtle, Pelodiscus sinensis
作者：Wang, Fen;Chen, Zhu;Cheng, Yunsheng;Hou, Guanjun;Ji, Suofei;Song, Guangtong;Jiang, Yelin;Wang, Fen;Chen, Zhu;Cheng, Yunsheng;Hou, Guanjun;Ji, Suofei;Zhu, Chengjun;Song, Guangtong;Jiang, Yelin;Wang, Fen;Cheng, Yunsheng;Hou, Guanjun;Ji, Suofei;Song, Guangtong;Jiang, Yelin;Zhang, Ye;Li, Jie;Wu, Yuchun
关键词：Pelodiscus sinensis; Yangtze strain; Japanese strain; Proximate composition; Amino acids; Fatty acids
