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Identification and immunoregulatory role of cathepsin A in the red swamp crayfish, Procambarus clarkii

文献类型： 外文期刊

作者： Huang, Long 1 ; Dai, Li-Shang 1 ; Wu, Ben-Li 1 ; Zhang, Ye 1 ; Chen, Jing 1 ; He, Ji-Xiang 1 ;

作者机构： 1.Anhui Acad Agr Sci, Fishery Inst, Anhui Prov Key Lab Aquaculture & Stock Enhanceme, Hefei 230031, Peoples R China

2.Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Peoples R China

关键词： Crustaceans; RNA interference; Innate immunity; Microbial infection; Immune defence

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2020 年 153 卷

页码：

收录情况： SCI

摘要： Cathepsins are a group of lysosomal hydrolytic enzymes, broadly distributed in animals, and regulate various physiological processes. However, the immune functions of cathepsins are poorly understood in invertebrates. Therefore, to further provide information about the importance of cathepsins in the innate immune system of crustaceans, cathepsin A from Procambarus clarkii (Pc-cathepsin A) was characterized and its distribution in different tissues was determined. The immunological functions of the Pc-cathepsin A were also evaluated. The Pc-cathepsin A showed high sequence homology to cathepsins of other species, as it contained serine and histidine active sites. Quantitative RT-PCR analysis revealed that the expression of Pc-cathepsin A was highest in the gill, gut, and the hepatopancreas, with variable amounts in the muscle, stomach, heart, and hemocytes. The mRNA expression of Pc-cathepsin A was significantly increased in hepatopancreas challenged with lipopolysaccharide (LPS), peptidoglycan (PGN), and polycytidylic acid (poly I:C). The results of an in vivo analysis revealed that Pc-cathepsin A knockdown by double-stranded RNA in P. clarkii modulated the expression of immune-pathway associated genes in hepatopancreas. Collectively, these results suggest that Pc-cathepsin A modulates innate immune responses by affecting the expression of immune-pathway associated genes, thus revealing a regulatory link between Pc-cathepsin A and immune pathways in P. clarkii, and that Pc-cathepsin A plays an essential biological role in the immune defence against microbial pathogens. (C) 2020 Published by Elsevier B.V.

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