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Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway

文献类型： 外文期刊

作者： Sun, Chuanbo 1 ; Xu, Yuting 3 ; Xu, Guangpei 1 ; Ji, Xu 2 ; Jiang, Ping 1 ; He, Yanfei 1 ;

作者机构： 1.West Anhui Univ, Coll Biotechnol & Pharmaceut Engn, Luan 237012, Peoples R China

2.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Anhui Prov Key Lab Livestock & Poultry Prod Safety, Hefei 230001, Peoples R China

3.Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Peoples R China

关键词： Active fractions; Jingfang Baidu powder; Klebsiella; Pneumonia

期刊名称：JOURNAL OF ETHNOPHARMACOLOGY （ 影响因子：5.4； 五年影响因子：5.3 ）

ISSN： 0378-8741

年卷期： 2024 年 330 卷

页码：

收录情况： SCI

摘要： Ethnopharmacological relevance: Jingfang Baidu Powder (JFBDP) is a classic traditional Chinese medicine prescription. Although Jingfang Baidu powder obtained a general consensus on clinical efficacy in treating pneumonia, there were many Chinese herbal drugs in formula, complex components, and large oral dosage, which brings certain obstacles to clinical application. Aim of the study: Therefore, screening of the active fraction that exerts anti -pneumonia helps improve the pharmaceutical preparation, improve the treatment compliance of patients, and further contribute to the clinical application, and the screening of the new active ingredients with anti -pneumonia. The histopathological observation, real-time quantitative PCR, western blotting, and immunofluorescence were applied to evaluate the anti -pneumonia efficacy of active fractions from JFBDP. Results: Three fractions from JFBDP inhibit the gene expression of IL-1 beta, IL -10, CCL3, CCL5, and CCL22 in lung tissue infected by Klebsiella at various degrees, and presented a good dose -response relationship. JF50 showed stronger anti-inflammatory effects among three fractions including JF30, JF50, and JF75. Besides, JF50 significantly reduced the protein expression of TLR4 and Myd88 in lung tissue infected with Klebsiella, and it also significantly inhibited p-ERK and p-NF-kappa B p65. JF50 significantly inhibits the protein expression of Caspase 3, Caspase 8, and Caspase 9 in lung tissue infected with Klebsiella at the dose of 25 mg/kg and 50 mg/kg. Conclusion: JF50 improves lung pathological damage in Klebsiella pneumonia mice by inhibiting the TLR4/ Myd88/NF-kappa B-ERK signaling pathway, and inhibiting apoptosis of lung tissue cells. These findings provide a reference for further exploring the active substance basis of Jingfang Baidu Powder in treating bacterial pneumonia.

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