Complementary transcriptomic and proteomic analyses elucidate the toxicological molecular mechanisms of deoxynivalenol-induced contractile dysfunction in enteric smooth muscle cells
文献类型： 外文期刊
作者： Qiao, Yu 1 ; Ji, Xu 2 ; Guo, Huiduo 3 ; Zheng, Weijiang 1 ; Yao, Wen 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Anim Sci & Technol, .1 Weigang Rd, Nanjing 210095, Peoples R China
2.Anhui Acad Agr Sci, Inst Anim Sci & Vet Med, Anhui Prov Key Lab Livestock & Poultry Prod Safety, Hefei 230031, Peoples R China
3.Jiangsu Univ Sci & Technol, Coll Biotechnol, Zhenjiang 212018, Peoples R China
4.Nanjing Agr Univ, Key Lab Anim Physiol & Biochem, Minist Agr & Rural Affairs Peoples Republ China, Nanjing 210095, Peoples R China
关键词： Deoxynivalenol; Enteric smooth muscle contractility; ECM-Receptor interaction; Actin polymerization; Transcriptomic; Proteomic
期刊名称：FOOD AND CHEMICAL TOXICOLOGY （ 影响因子：4.3； 五年影响因子：5.1 ）
ISSN： 0278-6915
年卷期： 2024 年 186 卷
页码：
收录情况： SCI
摘要： Deoxynivalenol (DON) is one of the frequent Fusarium mycotoxins and poses a serious threat to public health worldwide. DON-induced weight loss is tightly connected with its ability to decrease feed intake by influencing gastrointestinal tract (GIT) motility. Our previous reports indicated that DON interfered with intestinal motility by injuring the contractility of enteric smooth muscle cells (SMC). Here, we further explored the potential mechanisms by employing a complementary method of transcriptomics and proteomics using the porcine enteric smooth muscle cell line (PISMC) as an experimental model. The transcriptomic and proteomic data uncover that the expression of numerous extracellular matrix (ECM) proteins and multiple integrin subunits were downregulated in PISMC under DON exposure, suppressing the ECM-integrin receptor interaction and its mediated signaling. Furthermore, DON treatment could depress actin polymerization, as reflected by the upregulated expression of Rho GTPase-activating proteins and cofilin in PISMC. Meanwhile, the expression levels of downstream contractile apparatus genes were significantly inhibited after challenge with DON. Taken together, the current results suggest that DON inhibits enteric SMC contractility by regulating the ECM-integrin-actin polymerization signaling pathway. Our findings provide novel insights into the potential mechanisms behind the DON toxicological effects in the GIT of humans and animals.
- 相关文献
作者其他论文 更多>>
-
A comparative analysis of carcass traits and meat quality between Tibetan sheep and their three-way crossbred sheep
作者：Qiao, Yu;Tang, Jishun;Zhang, Wei;Chen, Sheng;Hui, Wenqiao;Yang, Deming
关键词：crossbred lamb; Tibetan sheep; muscle development; meat flavor; differentially expressed genes
-
Berberine alleviates enterotoxigenic Escherichia coli-induced intestinal mucosal barrier function damage in a piglet model by modulation of the intestinal microbiome
作者：Du, Min;Liu, Xinran;Wang, Yue;Liu, Xiaodan;Zhao, Chunfang;Jin, Erhui;Gu, Youfang;Wang, Hongyu;Zhang, Feng;Ji, Xu;Zhao, Chunfang;Jin, Erhui;Gu, Youfang;Zhang, Feng
关键词：enterotoxigenic Escherichia coli; berberine; weaned piglet; intestinal mucosal barrier function; intestinal microbiome
-
Taurine ameliorates deoxynivalenol-induced intestinal injury in piglets: Restoration of mitochondrial function linked to the PGC1α-NRF1/2 axis
作者：Ji, Xu;Ding, Hongyan;Zhou, Fen;Wu, Dong;Zhang, Feng;Zhang, Feng
关键词：Deoxynivalenol; Taurine; Intestinal injury; Mitochondria function; PGC1 alpha-NRF1/2 axis; Piglet model
-
Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway
作者：Sun, Chuanbo;Xu, Guangpei;Jiang, Ping;He, Yanfei;Ji, Xu;Xu, Yuting
关键词：Active fractions; Jingfang Baidu powder; Klebsiella; Pneumonia
-
Berberine alleviates ETEC-induced intestinal inflammation and oxidative stress damage by optimizing intestinal microbial composition in a weaned piglet model
作者：Wang, Yue;Zhang, Ziting;Du, Min;Liu, Xiaodan;Zhao, Chunfang;Pang, Xunsheng;Jin, Erhui;Wen, Aiyou;Li, Shenghe;Zhang, Feng;Ji, Xu;Zhao, Chunfang;Jin, Erhui;Li, Shenghe;Zhang, Feng
关键词：enterotoxigenic Escherichia coli; berberine; weaned piglet; intestinal inflammation; oxidative damage
-
New insights into the unfolded protein response (UPR)-anterior gradient 2 (AGR2) pathway in the regulation of intestinal barrier function in weaned piglets
作者：Zhang, Feng;Chen, Mengxian;Liu, Xiaodan;Li, Shenghe;Jin, Erhui;Zhang, Feng;Li, Shenghe;Jin, Erhui;Ji, Xu
关键词：Weaned piglet; Endoplasmic reticulum stress; Unfolded protein response; Anterior gradient 2; Intestinal barrier function
-
Ionomics-metabolome association analysis as a new approach to the impact of dietary copper levels in suckling piglets model
作者：Zhang, Feng;Liu, Xiaodan;Jin, Erhui;Zhang, Feng;Jin, Erhui;Yao, Wen;Yao, Wen;Ji, Xu;Jin, Erhui
关键词：
