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Optimization of water-soluble polysaccharides from stem lettuce by response surface methodology and study on its characterization and bioactivities

文献类型: 外文期刊

作者: Nie, Chenzhipeng 1 ; Zhu, Peilei 1 ; Wang, Mingchun 1 ; Ma, Shuping 1 ; Wei, Zihao 4 ;

作者机构: 1.Anhui Agr Univ, Anhui Prov Agr Prod Proc Engn Lab, Hefei 230036, Anhui, Peoples R China

2.Anhui Agr Univ, Dept Food Sci & Engn, Hefei 230036, Anhui, Peoples R China

3.Anhui Acad Agr Sci, Inst Hort, Hefei 230031, Anhui, Peoples R China

4.Rutgers State Univ, Dept Food Sci, 65 Dudley Rd, New Brunswick, NJ 08901 USA

关键词: Stem lettuce;Polysaccharide;Response surface methodology;Characterization;Antioxidant;alpha-Amylase

期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:6.953; 五年影响因子:6.737 )

ISSN:

年卷期:

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收录情况: SCI

摘要: Stem lettuce is widely consumed as a vegetable in China. It is also used as a traditional Chinese medicine for a long time. Up to now, no information is available for stem lettuce polysaccharide (SLP). In this study, extraction optimization, characterization, and antioxidant activity of SLP was investigated.The maximum SLP yield of 20.89% was obtained under the optimal extraction conditions as follows: extraction temperature 95 degrees C, extraction time 3.3 h and ratio of water to material 25 mL/g. The sulfate content of SLP was 5.82% and the main monosaccharides were GalA, Gal, and Ara with a mole ratio of 49.2: 23.3: 22.9. FTIR spectrum and HPGPC result further indicated that SLP is a sulfated polydisperse heterpolysaccharide. Congo-red test and AFM scan indicated that SLP might be branching and intertwining structure with triple helix conformation. Moreover, SLP exhibited potent antioxidant activity and alpha-amylase inhibitory activity, hence it could be used as a potential natural antioxidant and hypoglycemic agent in medicine or functional food fields. (C) 2017 Elsevier B.V. All rights reserved.

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