您好，欢迎访问安徽省农业科学院 机构知识库！

Functional Characterization of Laccase Isozyme (PoLcc1) from the Edible Mushroom Pleurotus ostreatus Involved in Lignin Degradation in Cotton Straw

文献类型： 外文期刊

作者： Li, Guoqing 1 ; Wang, Yahui 1 ; Zhu, Peilei 1 ; Zhao, Guiyun 1 ; Liu, Caiyu 1 ; Zhao, Hongyuan 1 ;

作者机构： 1.Anhui Acad Agr Sci, Inst Hort Res, State Key Lab Hort Crop Germplasm Resources Creat, Minist Agr & Rural Affairs, Hefei 230031, Peoples R China

2.Anhui Agr Univ, Coll Life Sci, Hefei 230036, Peoples R China

3.Prov Resource Database Wood Rot Edible Mushrooms, Hefei 230031, Peoples R China

关键词： Pleurotus ostreatus; laccase; Lacc1; cotton stalk; lignin degradation; functional groups

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 21 期

页码：

收录情况： SCI

摘要： Fungal laccases play important roles in the degradation of lignocellulose. In this study, the laccase producing cotton straw medium for Pleurotus ostreatus was optimized by single-factor and orthogonal experiments, and to investigate the role of Lacc1 gene, one of the laccase-encoding genes, in the degradation of cotton straw lignin, an overexpression strain of Lacc1 gene was constructed, which was analyzed for the characteristics of lignin degradation. The results demonstrated that the culture conditions with the highest lignin degradation efficiency of the P. ostreatus were the cotton straw particle size of 0.75 mm, a solid-liquid ratio of 1:3 and containing 0.25 g/L of Tween in the medium, as well as an incubation temperature of 26 degrees C. Two overexpression strains (OE L1-1 and OE L1-4) of Lacc1 gene were obtained, and the gene expression increased 12.08- and 33.04-fold, respectively. The results of H-1-NMR and FTIR analyses of significant changes in lignin structure revealed that Lacc1 gene accelerated the degradation of lignin G-units and involved in the cleavage of beta-O-4 linkages and the demethylation of lignin units. These findings will help to improve the efficiency of biodelignification and expand our understanding of its mechanism.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：安徽省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台