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MicroRNA profiling of the intestinal tissue of Kazakh sheep after experimental Echinococcus granulosus infection, using a high-throughput approach

文献类型： 外文期刊

作者： Jiang, Song 1 ; Li, Xin 1 ; Wang, Xuhai 1 ; Ban, Qian 3 ; Hui, Wenqiao 1 ; Jia, Bin 1 ;

作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, Rd Beisi, Shihezi 832003, Xinjiang, Peoples R China

2.Anhui Acad Agr Sci, Inst Anim Husb & Vet Med, Rd Nongkenan, Hefei 230031, Anhui, Peoples R China

3.Anhui Univ, Sch Life Sci, Ctr Stem Cell & Translat Med, Rd Jiulong, Hefei 230000, Anhui, Peoples R China

关键词： microRNA;Cystic Echinococcosis;Sheep;MHC haplotype

期刊名称：PARASITE （ 影响因子：3.0； 五年影响因子：3.046 ）

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收录情况： SCI

摘要： Cystic echinococcosis (CE), caused by infection with the larval stage of the cestode Echinococcus granulosus, is a chronic zoonosis, to which sheep are highly susceptible. Previously, we found that Kazakh sheep with different MHC haplotypes differed in CE infection. Sheep with haplotype MHCMvaIbc-SacIIab-Hin1Iab were resistant to CE infection, while their counterparts without this haplotype were not. MicroRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at the post-transcriptional level and play essential roles in fundamental biological processes such as development and metabolism. To identify microRNA controlling resistance to CE in the early stage of infection, microRNA profiling was conducted in the intestinal tissue of sheep with resistant and non-resistant MHC haplotypes after peroral infection with E. granulosus eggs. A total of 351 known and 186 novel miRNAs were detected in the resistant group, against 353 known and 129 novel miRNAs in the non-resistant group. Among these miRNAs, 83 known miRNAs were significantly differentially expressed, including 75 up-regulated and 8 down-regulated miRNAs. Among these known microRNAs, miR-21-3p, miR-542-5p, miR-671, miR-134-5p, miR-26b, and miR-27a showed a significantly higher expression in CE-resistant sheep compared to the CE-non-resistant library, with the FC > 3. Functional analysis showed that they were NF-kB pathway-responsive miRNAs, which are involved in the inflammation process. The results suggest that these microRNAs may play important roles in the response of intestinal tissue to E. granulosus.

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