BmADARa cooperatively inhibits BmNPV proliferation through the interaction of its dsRBD2 with BmDcr-2-DEXHc in silkworm, Bombyx mori
文献类型： 外文期刊
作者： Jiang, Song 1 ; Ye, Chong-Jun 2 ; Wu, Yu-Chen 1 ; Shi, Ruo-Yun 1 ; Yu, Yu-Long 1 ; Saneela, Syeda 1 ; Liang, Dan 3 ; Huang, Yan-Jiao 1 ; Shi, Xia-Ming 1 ; Meng, Yan 1 ;
作者机构： 1.Anhui Agr Univ, Sch Life Sci, 130 West Changjiang Rd, Hefei 230036, Peoples R China
2.Anhui Acad Agr Sci, Hefei, Peoples R China
3.Yangzhou Univ, Coll Biol Sci & Technol, Yangzhou, Jiangsu, Peoples R China
4.Anhui Prov Key Lab Resource Insect Biol & Innovat, Hefei, Peoples R China
5.Anhui Int Joint Res & Dev Ctr Sericulture Resource, Hefei, Peoples R China
6.Zhejiang Univ, Sir Run Run Shaw Hosp, Canc Ctr, Sch Med,Dept Med Oncol,Zhejiang Key Lab Multi Prec, Hangzhou, Peoples R China
关键词： BmADARa; BmDcr-2; BmNPV proliferation; RNAi pathway; silkworm
期刊名称：INSECT SCIENCE （ 影响因子：3.0； 五年影响因子：3.5 ）
ISSN： 1672-9609
年卷期： 2025 年
页码：
收录情况： SCI
摘要： Adenosine deaminases that act on RNA (ADARs) are RNA editing enzymes capable of converting adenosine into inosine at specific sites within double-stranded RNA (dsRNA), widely distributed across various animal species. Dicer (Dcr), a member of the RNase III family and a crucial component of the RNA-induced silencing complex (RISC), allows ADAR to participate in innate immunity through Dcr-2 in Drosophila. Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the viruses that can cause substantial economic losses to the sericulture industry upon infecting silkworm. Knocking down the expression of BmDcr-2 in silkworm enhances the proliferation of BmNPV. Our previous research revealed the existence of a predominantly expressed subtype, ADARa, in silkworm (BmADARa), which shares homology with Drosophila ADAR. It remains unclear whether BmADARa can also participate in innate immunity through BmDcr-2. Initially, through bacterial challenge experiments, we found that BmADARa exhibited the highest responsiveness to BmNPV stimulation. Further studies demonstrated that BmADARa, in conjunction with BmDcr-2-DEXHc (DEAD-box helicase domain), collectively inhibits the proliferation of BmNPV. BmADARa interacts with the DEXHc domain of BmDcr-2 through its dsRNA binding domain 2 (dsRBD2), thereby enhancing its ability to inhibit BmNPV proliferation. These results lay a foundation for the study of the function and molecular mechanism of BmADARa in innate immunity, and provide a new experimental ideas for antiviral research in B. mori.
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