Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency
文献类型： 外文期刊
作者： Ban, Qian 1 ; Hui, Wenqiao 3 ; Duan, Quanchao 1 ; Liu, Wenfei 4 ; Zhang, Meng 1 ; Li, Xiaofeng 1 ; Kang, Hanyue 2 ; Shi, Zhenni 1 ; Zhang, Yan 1 ; Hou, Jinbin 1 ; Xu, Xiuzhen 2 ; Ye, Shoudong 1 ; Zhang, Baowei 1 ; Xu, Xiaobin 2 ;
作者机构： 1.Anhui Univ, Ctr Stem Cell & Translat Med, Sch Life Sci, Hefei 230601, Anhui, Peoples R China
2.Tongji Univ, Sch Mat Sci & Engn, Key Lab Adv Civil Engn Mat, Key Lab D&A Met Funct Mat,Minist Educ, Shanghai 201804, Peoples R China
3.Anhui Acad Agr Sci, Inst Anim Husb & Vet Med, Anhui Prov Key Lab Livestock & Poultry Prod Safety, Hefei 230031, Anhui, Peoples R China
4.Univ Calif Los Angeles, Calif Nanosyst Inst, Los Angeles, CA 90095 USA
5.Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
期刊名称：ISCIENCE （ 影响因子：5.8； 五年影响因子：5.8 ）
ISSN：
年卷期： 2023 年 26 卷 4 期
页码：
收录情况： SCI
摘要： Here, we report the scalable fabrication of 2i-functionalized micro-pyramid-array (mu PyA/+2i) inserts for use in commercial multi-well plates, as the alternative culti-vation platform for maintaining long-term self-renewal and pluripotency of mul-tiple mESCs and mouse induced pluripotent stem cells. Relevant evidence including cell morphology characterization increased alkaline phosphatase activ-ity, high expression of mESC self-renewal markers, decreased levels of differen-tiation-associated markers, and high proportion of self-renewal marker cells are provided. Further studies demonstrated that mu PyA/+2i could cause a higher cell density in mESC colony, and induce gene expression changes. Subsequent studies showed that mu PyA/+2i can influence the cytoskeleton and promote cell adhesion through Cldn-7 upregulation. In summary, these mu PyA/+2i inserts offer flexible and gelatin-free micro-envriomnets to maintain long-term self-renewal and pluripotency of mESCs. Enabled by the microstructured inserst, the facile stem cell manipulation and transfer among culture dishes will broaden stem cells both in routine and translational applications.
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