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Isolation and characterization of three cadmium-inducible promoters from Oryza sativa

文献类型： 外文期刊

作者： Qiu, Chun-Hong 1 ; Li, Hao 1 ; Li, Juan 1 ; Qin, Rui-Ying 1 ; Xu, Rong-Fang 1 ; Yang, Ya-Chun 1 ; Ma, Hui 1 ; Song, Feng- 1 ;

作者机构： 1.Anhui Acad Agr Sci, Rice Res Inst, Key Lab Rice Genet Breeding Anhui Prov, Hefei 230031, Peoples R China

2.Anhui Acad Agr Sci, Rice Res Inst, Key Lab Rice Genet Breeding Anhui P

关键词： Cadmium stress;Rice;Inducible promoter;Heavy metals

期刊名称：JOURNAL OF BIOTECHNOLOGY （ 影响因子：3.307； 五年影响因子：3.778 ）

ISSN：

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收录情况： SCI

摘要： Cadmium (Cd) is an important soil pollutant. Developing genetically engineered crops might be a feasible strategy for Cd decontamination and damage prevention. Both genes and promoters are critical for the effective construction of genetically modified plants. Although many functional genes for Cd tolerance and accumulation have been identified, few reports have focused on plant Cd-inducible promoters. Here, we identified three Cd-inducible genes in the rice genome: two tau class glutathione S-transferase (GSTU) genes, OsGSTU5 and OsGSTU37, and an HSP20/alpha crystallin family protein gene, OsHSP18.6. The promoter sequences were isolated and tested in transgenic rice lines using a GUSplus reporter gene. All of the promoters exhibited low background expression under normal conditions and could be strongly induced by Cd stress. Although their strength was comparable to that of the constitutive OsACTIN promoter under Cd stress, their time-dependent expression patterns under both short- and long-term Cd exposure were markedly different. The responses of the three promoters to other heavy metals were also examined. Furthermore, heavy metal-responsive cis elements in the promoters were computationally analyzed, and regions determining the Cd stress response were analyzed using a series of truncations. Our results indicate that the three Cd-inducible rice promoters described herein could potentially be used in applications aimed at improving heavy metal tolerance in crops or for the bio-monitoring of environmental contamination. (C) 2015 Elsevier B.V. All rights reserved.

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