The efficacy of chimeric vaccines constructed with PEP-1 and Ii-Key linking to a hybrid epitope from heterologous viruses
文献类型： 外文期刊
作者： Liu, Xue-lan 1 ; Shan, Wen-jie 1 ; Xu, Shan-shan 1 ; Zhang, Jin-jing 1 ; Xu, Fa-zhi 1 ; Xia, Sheng-lin 2 ; Dai, Yin 3 ;
作者机构： 1.Anhui Agr Univ, Key Lab Zoonoses Anhui Prov, Hefei 230036, Anhui, Peoples R China
2.Ctr Anim Epidem Dis Prevent & Control, Wuhu 241000, Anhui, Peoples R China
3.Anhui Acad Agr Sci, Anhui Anim Husb & Vet Res Inst, Hefei 230031, Anhui, Peoples R China
关键词： li-Key;PEP-1;Heterologous epitope-peptide;The vehicle effect;Vaccine
期刊名称：BIOLOGICALS （ 影响因子：1.856； 五年影响因子：2.05 ）
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收录情况： SCI
摘要： The heterologous epitope-peptide from different viruses may represent an attractive candidate vaccine. In order to evaluate the role of cell-permeable peptide (PEP-1) and Ii-Key moiety from the invariant chain (Ii) of MHC on the heterologous peptide chimeras, we linked the two vehicles to hybrid epitopes on the VP2 protein (aa197-209) of the infectious bursal disease virus and HN protein (aa345-353) of the Newcastle disease virus. The chimeric vaccines were prepared and injected into mice. The immune effects were measured by indirect ELISA. The results showed that the vehicle(s) could significantly boost immune effects against the heterologous epitope peptide. The Ii-Key-only carrier induced more effective immunological responses, compared with the PEP-1 and li-Key hybrid vehicle. The carrier peptide hybrids all showed strong colocalization with major histocompatibility complex (MHC) class II molecules compared with the epitope-peptide (weakly-binding) after co-transfection into 293T cells. Together, our results lay the groundwork for designing new hybrid vaccines based on Ii-Key and/or PEP-1 peptides. (C) 2015 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.
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