Affinity purification and characterisation of zinc chelating peptides from rapeseed protein hydrolysates: Possible contribution of characteristic amino acid residues
文献类型： 外文期刊
作者： Xie, Ningning 1 ; Huang, Jingjing 1 ; Li, Bo 2 ; Cheng, Jianghua 1 ; Wang, Zhuochen 1 ; Yin, Junfeng 1 ; Yan, Xiaoming 1 ;
作者机构： 1.Anhui Acad Agr Sci, Inst Agrofood Sci & Technol, Hefei 230031, Peoples R China
2.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
3.Minist Educ, Key Lab Funct Dairy, Beijing 100083, Peoples R China
关键词： Zinc;Rapeseed protein hydrolysates;Chelating peptides;Immobilized metal affinity chromatography;Alcalase
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Zinc is an essential trace element for human growth and development. In this work, zinc-chelating peptides from rapeseed protein hydrolysates produced with alcalase were investigated by affinity chromatography with immobilized zinc and Sephadex G-25 gel filtration. Four small peptides, namely, Ala-Arg, Asn-Ser-Met (NSM), Gly-Lys-Arg, and Glu-Pro-Ser-His, were obtained and identified by reversed-phase high-performance liquid chromatography and electrospray ionization mass spectrometry. The zinc-chelating ability of the four peptides was further validated by inductively coupled plasma atomic emission spectrometry (ICP-AES). NSM was found to exhibit the highest zinc-chelating rate, which was better than that of reduced glutathione. We speculated that the Asn residue at the amino-terminus might facilitate this zinc-chelating ability. Therefore, utilizing small peptides from rapeseed protein as novel carriers for zinc supplement was feasible. (C) 2014 Elsevier Ltd. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
Study on the microbial mechanisms of enhancing Agaricus bisporus growth through inoculation with Pseudomonas putida
作者：Li, Hongtu;Liu, Lanhua;Xu, Boyang;Wu, Xunfeng;Mu, Dongdong;Li, Xingjiang;Yan, Xiaoming;Chen, Qun;Wu, Xiaolei
关键词：Agaricus bisporus; Pseudomonas putida; Microbial communities; Microbial cooperation; Microbial agent; Microbial ecological networks
-
A novel zinc-chelating peptide identified from rapeseed (Brassica napus) protein hydrolysate: insights into its zinc-binding sites by density functional theory
作者：Wang, Hao;Huang, Jingjing;Yang, Mingliu;Zhou, Yingqin;Yin, Junfeng;Yan, Yan;Xie, Ningning;Wang, Hao;Huang, Jingjing;Yang, Mingliu;Zhou, Yingqin;Yin, Junfeng;Yan, Yan;Xie, Ningning;Wang, Hao
关键词：Binding site; geometric structure; quantum chemistry; rapeseed protein; zinc chelating peptide
-
Responses of Soil Labile Organic Carbon on Aggregate Stability across Different Collapsing-Gully Erosion Positions from Acric Ferralsols of South China
作者：Tang, Xian;Li, Bo;Lu, Ying;Tang, Xian;Zhao, Jianrong;Wang, Hong;Alhaj Hamoud, Yousef;Shaghaleh, Hiba;Wang, Jiajia;Zhao, Tao
关键词：soil LOC; C pool management index; collapsing gully erosion; aggregate stability; Acric Ferralsols
-
Physicochemical characteristics and gel-forming properties of mandarin fish (Siniperca chuatsi) protein during the fish fermentation with Lactobacillus sake SMF-L5: The formation of garlic-cloves shaped protein gel
作者：Zhou, Yingqin;Yang, Mingliu;Yin, Junfeng;Huang, Jingjing;Yan, Yan;Zhang, Fusheng;Xie, Ningning;Zhou, Yingqin;Yin, Junfeng;Huang, Jingjing;Yan, Yan;Zhang, Fusheng;Xie, Ningning;Yang, Mingliu
关键词：Mandarin fish; Fermented fish; Physicochemical characteristic; Elastic texture; Pearson correlation
-
Processing quality and aroma characteristics of fresh noodles intermingled with large-leaf yellow tea powder
作者：Wang, Zhuochen;Li, Yuhuan;Jiang, Jian;Wang, Zhuochen;Jiang, Jian;Li, Yuhuan;Qian, Chao;Xiong, Guoyuan;Chen, Qi;Chen, Qi;Feng, Bin;Chen, Qi
关键词：Fresh noodles; Tea polyphenols; Antioxidant; Gas chromatography -ion mobility spectrometry; (GC-IMS); Flavor
-
Combining gas chromatography-ion mobility spectrometry and olfactory analysis to reveal the effect of filled-N2 anaerobic treatment duration on variation in the volatile profiles of gabaron green tea
作者：Huang, Dongzhu;Li, Mengru;Fu, Maoyin;Wan, Xiaochun;Chen, Qi;Wang, Hong;Chen, Qi;Hu, Shaode;Wang, Zhuochen
关键词：Gabaron green tea; Volatile compounds; Anaerobic treatment; Aroma extract dilution analysis
-
Calcium dynamics during the growth of Agaricus bisporus: implications for mushroom development and nutrition
作者：Wu, Juan;Wang, Rui;Liu, Xue;Ni, Ying;Sun, Hui;Deng, Xiaonan;Wan, Lingzhong;Liu, Fangzhi;Yan, Xiaoming;Tang, Jun;Yu, Junjie
关键词：Agaricus bisporus; Growth process; Calcium content and forms
