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Magnetic graphene-enhanced exonuclease III assisted amplification strategy driven carbon nanozyme for tri-mode detection of Escherichia coli O157:H7

文献类型： 外文期刊

作者： Ren, Yuwei 1 ; Cao, Lulu 1 ; Li, Zongyang 1 ; Zhang, Xin 1 ; Li, Hui 1 ; Jiao, Rui 1 ; Yu, Xiaoyu 1 ; Liang, Zhao 1 ; Li, Ganghui 1 ; Xia, Xiyang 1 ; Ling, Na 1 ; Zhang, Danfeng 1 ; Cheng, Jianghua 2 ; Ye, Yingwang 1 ;

作者机构： 1.Hefei Univ Technol, Engn Res Ctr Bioproc, Sch Food & Biol Engn, Key Lab Anim Food Green Mfg & Resource Min Anhui P, Hefei 230009, Peoples R China

2.Anhui Acad Agr Sci, Inst Agroprod Proc, Anhui Engn Lab Food Microbial Fermentat & Funct Ap, Hefei 230036, Peoples R China

关键词： Tri-mode detection; Escherichia coli O157:H7; Cu-cl co-doped nanozyme; Cascade signal amplification; Deep learning

期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）

ISSN： 0308-8146

年卷期： 2025 年 471 卷

页码：

收录情况： SCI

摘要： Ultra-precision point-of-care detection of Escherichia coli O157:H7 in foods is an important issue. Here, the detection sensitivity was improved by a signal cascade amplification strategy synergised by exonuclease III assisted isothermal amplification and reverse magnetic strategy. The double-stranded DNA formed by the aptamer and the target DNA as a sensing switch, avoiding the complex process of specific nucleic acid extraction. Further, the signal display element is a green nanozyme synthesized by co-doping Cu and Cl elements with lignin as precursor, which has excellent peroxidase activity and fluorescence properties. The detection limits of fluorescence, colorimetric, and photothermal modes were 6.1, 8.4, and 9.7 cfu/mL, respectively. In order to enhance the precision of cascade signal amplification and the portability of quantitative detection, we built a neural network BP model installed in a smartphone by measuring multi-component data, which provides flexible and selectable application scenarios for the on-site detection of foodborne pathogens.

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