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Nondestructive determination of transgenic Bacillus thuringiensis rice seeds (Oryza sativa L.) using multispectral imaging and chemometric methods

文献类型： 外文期刊

作者： Liu, Changhong 1 ; Liu, Wei 2 ; Lu, Xuzhong 3 ; Chen, Wei 1 ; Yang, Jianbo 3 ; Zheng, Lei 1 ;

作者机构： 1.Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China

2.Hefei Univ, Intelligent Control & Compute Vis Lab, Hefei 230601, Peoples R China

3.Anhui Acad Agr Sci, Rice Res Inst, Hefei 230031, Peoples R China

4.Hefei Univ Technol, Sch Med Engn, Hefei 230009, Peoples R China

关键词： Multispectral imaging;Rice seed;Transgenic;Nondestructive determination;Chemometric

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

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收录情况： SCI

摘要： Crop-to-crop transgene flow may affect the seed purity of non-transgenic rice varieties, resulting in unwanted biosafety consequences. The feasibility of a rapid and nondestructive determination of transgenic rice seeds from its non-transgenic counterparts was examined by using multispectral imaging system combined with chemometric data analysis. Principal component analysis (PCA), partial least squares discriminant analysis (PLSDA), least squares-support vector machines (LS-SVM), and PCA-back propagation neural network (PCA-BPNN) methods were applied to classify rice seeds according to their genetic origins. The results demonstrated that clear differences between non-transgenic and transgenic rice seeds could be easily visualized with the nondestructive determination method developed through this study and an excellent classification (up to 100% with LS-SVM model) can be achieved. It is concluded that multispectral imaging together with chemometric data analysis is a promising technique to identify transgenic rice seeds with high efficiency, providing bright prospects for future applications.

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