Oviductal sperm storage in the Chinese pond turtle, Mauremys reevesii, depends on androgen-based promotion of the BCL 2 anti-apoptotic pathway
文献类型： 外文期刊
作者： Chen, Wei 1 ; Wang, Yajie 1 ; Gong, Mengmeng 1 ; Gao, Hailin 1 ; Zhou, Huaxing 2 ; Bu, Xingjiang 1 ; Xia, Xingquan 1 ; Nie, Liuwang 1 ;
作者机构： 1.Anhui Normal Univ, Coll Life Sci, Anhui Prov Key Lab Conservat & Exploitat Biol Reso, Wuhu, Peoples R China
2.Anhui Acad Agr Sci, Fisheries Res Inst, Anhui Key Lab Aquaculture & Stock Enhancement, Hefei, Peoples R China
3.Anhui Normal Univ, Coll Life Sci, Wuhu 241000, Anhui, Peoples R China
关键词： androgen receptor; apoptosis; BAX; BCL 2; Mauremys reevesii; oviduct; reproductive tract; reptiles; sperm storage
期刊名称：REPRODUCTION FERTILITY AND DEVELOPMENT （ 影响因子：1.9； 五年影响因子：2.0 ）
ISSN： 1031-3613
年卷期： 2023 年 35 卷 6 期
页码：
收录情况： SCI
摘要： Context. Sperm storage is a complex and highly coordinated process that is regulated by a variety of factors. The BCL 2 protein family plays a key role in regulating apoptosis, and determines sperm survival. Aims. The objective of this study was to explore the correlation between sperm storage and the BCL 2 protein family in the oviduct of Mauremys reevesii. Methods. Hematoxylin-eosin (HE) staining, immunohistochemistry (IHC) and real-time quantitative polymerase chain reaction (RT-qPCR) techniques were used to investigate three parts of the reproductive tract (isthmus, uterus and vagina) of mated and unmated female M. reevesii. Key results. Hematoxylin-eosin staining revealed many sperm stored in the oviduct. IHC showed positive immunostaining for the BCL 2 and BAX proteins in epithelial ciliated and glandular cells. RT-qPCR indicated that the mRNA expressions of anti-apoptotic genes (BCL 2, MCL 1, BCL-W, BCL-XL) and the androgen receptor (AR) were significantly higher in mated turtles than unmated turtles. However, the expression of pro-apoptotic genes (BAX, BAD, BID and CASPASE 3) showed the opposite relationship. Conclusions. These results suggest that sperm entering the oviduct can promote the synthesis of anti-apoptotic genes to protect themselves from various degradation factors. Implications. These findings will help researchers understand the mechanisms of sperm storage.
- 相关文献
作者其他论文 更多>>
-
Differences in environmental microbial community responses under rice-crab co-culture and crab monoculture models under cyanobacterial bloom
作者：Tong, Li;Jun, Ling;He, Jiang;Min, Yang;Duan, Guoqing;Hu, Yuting;Zhou, Huaxing;Huan, Wang;Pan, Tingshuang;Tong, Li;Jun, Ling;He, Jiang;Min, Yang;Duan, Guoqing;Hu, Yuting;Zhou, Huaxing;Huan, Wang;Pan, Tingshuang
关键词：cyanobacterial blooms (CB); Chinese mitten crab; crab culture models; bacterial community; environmental factors; co-occurrence network; keystone taxa; carbon metabolism
-
The First Identification of Homomorphic XY Sex Chromosomes by Integrating Cytogenetic and Transcriptomic Approaches in Plestiodon elegans (Scincidae)
作者：Xu, Wannan;Li, Taiyue;Li, Jiahui;Liu, Siqi;Yu, Xing;Tang, Min;Dong, Jingxiu;Bu, Xingjiang;Xia, Xingquan;Nie, Liuwang;Xu, Wannan;Liu, Jianjun;Zhou, Huaxing
关键词：Plestiodon elegans; karyotype; RNAseq; sex markers; sex chromosomes
-
Effect of fasting and refeeding on serum physiology, intestinal microbiota, and transcriptome in Pseudobagrus vachellii
作者：Wang, Huan;Duan, Guoqing;Zhou, Huaxing;Hu, Yuting;Jiang, He;Wang, Huan;Duan, Guoqing;Zhou, Huaxing;Hu, Yuting;Jiang, He
关键词：DEG; Fasting; Liver; Microbiome; Pseudobagrus vachellii; Transcriptome
-
The 'Candidatus phytoplasma ziziphi' effectors SJP1 and SJP2 destabilise the bifunctional regulator ZjTCP7 to modulate floral transition and shoot branching
作者：Ma, Fuli;Huang, Xiang;Zhou, Junyong;Zhang, Ning;Deng, Mingsheng;Zheng, Yunyan;Zhao, Meiqi;Chen, Wei;Zhou, Wenmin;Zhai, Liping;Zhong, Lei;Pang, Kaixue;Liu, Xin;Zhong, Xinyue;Ren, Yifan;Liu, Yu;Sun, Jun;Zhou, Junyong;Sun, Qibao
关键词：flowering time; jujube witches' broom; SAP11 effector; shoot branching; Ziziphus jujuba
-
Genetic diversity of three consecutive selective breeding generations in Pseudobagrus vachellii (Actinopterygii: Siluriformes: Bagridae)
作者：Wang, Huan;Duan, Guoqing;Zhou, Huaxing;Wang, Huan;Duan, Guoqing;Zhou, Huaxing
关键词：genetic diversity; genetic structure; microsatellites; Pseudobagrus vachellii; selective breeding
-
Jujube witches' broom phytoplasmas inhibit ZjBRC1-mediated abscisic acid metabolism to induce shoot proliferation
作者：Ma, Fuli;Zhang, Shanqi;Yao, Yu;Chen, Mengting;Zhang, Ning;Deng, Mingsheng;Chen, Wei;Ma, Chi;Zhang, Xinyue;Guo, Chenglong;Huang, Xiang;Zhang, Zhenyuan;Li, Yamei;Li, Tingyi;Zhou, Junyong;Sun, Jun;Zhou, Junyong;Sun, Qibao;Yao, Yu;Li, Yamei
关键词：
-
Continuous identification of the tea shoot tip and accurate positioning of picking points for a harvesting from standard plantations
作者：Luo, Kun;Luo, Kun;Luo, Kun;Zhang, Xuechen;Cao, Chengmao;Qin, Kuan;Li, Weiqing;Chen, Le;Wu, Zhengmin;Wang, Chuan;Chen, Wei
关键词：tea shoots; continuous picking; two-dimensional imaging; low-damage picker; You Only Look Once
