A simple and affordable method of dna extraction from fish scales for polymerase chain reaction
文献类型： 外文期刊
作者： Li, Yanhe 1 ; Gul, Yasmeen 1 ; Gao, Zexia 1 ; Luo, Wei 1 ; Wang, Weimin 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China
2.Anhui Acad Agr Sci, Inst Fisheries, Hefei 230031, Peoples R China
3.COMSATS Inst Informat Technol, Dept Biosci, Sahiwal 57000, Pakistan
期刊名称：BIOCHEMICAL GENETICS （ 影响因子：1.89； 五年影响因子：1.959 ）
ISSN：
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收录情况： SCI
摘要： Most fish studies today are performed at the molecular level, which needs reliable, quick, and cost-effective DNA extraction protocols (Liu et al. 2009; Ming et al. 2010; Shen et al. 2010), Generally, the most widely used materials for DNA extraction in fish are fins or red blood cell samples (Yang et al. 2001; Liu et al. 2009; Zhang et al. 2009; Li et al. 2009). As fins are used by fish in locomotion, their removal would adversely impact survival of sampled fish. Similarly, collection of samples of enough red blood for DNA extraction from small fish would require them to be cultured further, and this would also be difficult for the researcher. Thus, simpler methods of sample collection are needed for DNA extraction.
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