Comparative analysis of different protocols for extraction of DNA from fish scales of Cyprinus carpio
文献类型： 外文期刊
作者： Li, Yanhe 1 ; Gul, Yasmeen 1 ; Cui, Lei 1 ; Cao, Xiaojuan 1 ; Wang, Weimin 1 ;
作者机构： 1.Huazhong Agr Univ, Minist Educ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan 430070, Peoples R China
2.Anhui Acad Agr Sci, Inst Fisheries, Hefei 230031, Peoples R China
3.COMSATS Inst Informat Technol, Dept Biosci, Sahiwal 57000, Pakistan
关键词： Cyprinus carpio;DNA extraction;fish scales;PCR
期刊名称：INDIAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.414； 五年影响因子：0.542 ）
ISSN： 0972-5849
年卷期： 2015 年 14 卷 3 期
页码：
收录情况： SCI
摘要： Four DNA extraction protocols from the fish scales of Cyprinus carpio were compared. The DNAs obtained from all the protocols were tested by PCR and the DNAs extracted using protocol 1 and 2 were also tested by restriction analysis. The results indicated that all four protocols extracted DNA suitable for PCR amplification. However, variations in the yield and purity of extracted DNA were observed between protocols. The DNA samples prepared by protocol 1 and 2 were amenable to restriction digestion by restriction enzyme HindIII and NotI. Different protocols have their own merits and shortcomings. Thus, the present study can be used as a reference for selecting suitable protocol according to the aim and need of the study undertaken.
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