文献类型： 外文期刊
作者： Li, Yanhe 1 ; Wang, Weimin 1 ; Liu, Xiaolian 1 ; Luo, Wei 1 ; Zhang, Jie 1 ; Gul, Yasmeen 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China
2.Anhui Acad Agr Sci, Inst Fisheries, Hefei 230031, Peoples R China
关键词： Crayfish exoskeleton;DNA amplification;DNA extraction
期刊名称：INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY （ 影响因子：0.818； 五年影响因子：1.006 ）
ISSN： 0019-5189
年卷期： 2011 年 49 卷 12 期
页码：
收录情况： SCI
摘要： Crayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-R) was used to amplify extracted DNA from CE, and compared to crayfish tail muscle DNA sample. Moreover, seven microsatellites markers were used to amplify the CE DNA samples set. Since the extracted DNA from CE is suitable for gene amplification, the results present usefulness of CE as an easy and convenient DNA source for PCR-based population genetic research.
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