The expression of prophenoloxidase mRNA in red swamp crayfish, Procambarus clarkii, when it was challenged
文献类型： 外文期刊
作者： Li, Yanhe 2 ; Deng, Wei 2 ; Yang, Keli 1 ; Wang, Weimin 2 ;
作者机构： 1.Huibei Acad Agr Sci, Inst Anim Husb & Vet, Huibei Key Lab Anim Embryo & Mol Breeding, Wuhan 430064, Peoples R China
2.Huazhong Agr Univ, Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Coll Fisheries, Wuhan 430070, Peoples R China
3.Anhui Acad Agr Sci, Inst Fisheries, Hefei 230031, Peoples R China
关键词： Aeromonas hydrophila;CpG oligodeoxynucleotide (ODN);Expression;Procambarus clarkii;Prophenoloxidase;White spot syndrome virus (WSSV)
期刊名称：GENOMICS （ 影响因子：5.736； 五年影响因子：4.939 ）
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收录情况： SCI
摘要： The expression of the prophenoloxidase (proPO) gene was investigated in nine tissues of red swamp crayfish Procambarus clarkii, by real-time PCR after challenges by CpG oligodeoxynucleotide (ODN), Aeromonas hydrophila and white spot syndrome virus (WSSV). The results can be summarized as follows: (i) the expression level of the proPO gene in haemocytes was highest among nine studied tissues before the challenge; (ii) the expression of proPO increased in all studied tissues after stimulation by CpG ODN and WSSV, and also increased in all tissues, except the ovary, after the A. hydrophila challenge; (iii) the whole expression profiles were different, suggesting that different immune mechanisms may exist for crayfish that are resistant to WSSV and A. hydrophila, although the expression in haemocytes was similar before and after the WSSV and A. hydrophila challenges.
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