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The Escherichia coli type III secretion system 2 Is involved in the biofilm formation and virulence of avian Pathogenic Escherichia coli

文献类型： 外文期刊

作者： Yin, Lei 1 ; Li, Qianwen 1 ; Wang, Zeping 1 ; Shen, Xuehuai 2 ; Tu, Jian 1 ; Shao, Ying 1 ; Song, Xiangjun 1 ; Qi, Kezong 1 ;

作者机构： 1.Anhui Agr Univ, Coll Anim Sci & Technol, Anhui Prov Key Lab Vet Pathobiol & Dis Control, Hefei 230036, Peoples R China

2.Anhui Acad Agr Sci, Livestock & Poultry Epidem Dis Res Ctr Anhui Prov, Inst Anim Husb & Vet Sci, Hefei 230031, Anhui, Peoples R China

关键词： Avian pathogenic Escherichia coli; ETT2; Biofilm formation; Virulence; Pathogenesis; RNA sequencing

期刊名称：COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES （ 影响因子：2.268； 五年影响因子：2.495 ）

ISSN： 0147-9571

年卷期： 2021 年 79 卷

页码：

收录情况： SCI

摘要： The Escherichia coli type III secretion system 2 (ETT2) is found in most pathogenic E. coli strains. Although many ETT2 gene clusters carry multiple genetic mutations or deletions, ETT2 is known to be involved in bacterial virulence. To date, no studies have been conducted on the role of ETT2 in the virulence of avian pathogenic Escherichia coli (APEC), which harbours ETT2. Thus, we deleted the ETT2 of APEC strain and evaluated the phenotypes and pathogenicities of the mutant. The results showed that deletion of ETT2 had no effect on APEC growth, but significantly promoted biofilm formation. In addition, as compared to the wild-type (WT) strain, the ETT2 deletion significantly promoted adherence to and invasion of DF-1 chicken fibroblasts and facilitated survival in the sera of specific-pathogen-free chickens. Analysis of the role of ETT2 in animal infection models demonstrated that the distribution of viable bacteria in the blood and organs of chicks infected with the Delta ETT2 was significantly higher than those infected with WT. The results of RNA sequencing indicated that multiple genes involved in biofilm formation, lipopolysaccharide components, fimbrial genes and virulence effector proteins are regulated by ETT2. Collectively, these results implicated ETT2 is involved in the biofilm formation and pathogenicity of APEC.

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