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Contiguous identity between entire coding regions of transgenic and native genes rather than special regions is essential for a strong co-suppression

文献类型： 外文期刊

作者： Chen, Yangyang 1 ; Ma, Shijie 2 ; Ku, Hangkai 1 ; Huangfu, Bingyuan 1 ; Wang, Kai 1 ; Du, Chang 1 ; Zhang, Meng 1 ;

作者机构： 1.Northwest A&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China

2.Anhui Acad Agr Sci, Crop Res Inst, Hefei 230031, Anhui, Peoples R China

3.South China Normal Univ, Coll Life Sci, Guangzhou 610631, Guangdong, Peoples R China

关键词： Continuous sequence identity; FAD2; FAE1; SiRNA; Small RNA; Strong co-suppression

期刊名称：PLANT SCIENCE （ 影响因子：5.2； 五年影响因子：5.7 ）

ISSN： 0168-9452

年卷期： 2024 年 341 卷

页码：

收录情况： SCI

摘要： The discovery of co-suppression in plants has greatly boosted the study of gene silencing mechanisms, but its triggering mechanism has remained a mystery. In this study, we explored its possible trigger mechanism by using Fatty acid desaturase 2 (FAD2) and Fatty acid elongase 1 (FAE1) strong co-suppression systems. Analysis of small RNAs in FAD2 co-suppression lines showed that siRNAs distributed throughout the coding region of FAD2 with an accumulated peak. However, mutations of the peak siRNA-matched site and siRNA derived site had not alleviated the co-suppression of its transgenic lines. Synthetic FAD2 (AtFAD2sm), which has synonymous mutations in the entire coding region, failed to trigger any co-suppression. Furthermore, 5 ' and 3 ' portions ofAtFAD2 and AtFAD2sm were swapped to form two hybrid genes, AtFAD2-3sm and AtFAD2-5sm. 80 % and 92 % of their transgenic lines exhibited co-suppression, respectively. Finally, FAE1s with different degrees of the continuous sequence identity compared with AtFAE1 were tested in their Arabidopsis transgenic lines, and the results showed the co-suppression frequency was reduced as their continuous sequence identity stepped down. This work suggests that contiguous identity between the entire coding regions of transgenic and native genes rather than a special region is essential for a strong co-suppression.

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