文献类型： 外文期刊

作者： Zhou, Qiumei ¹ ; Shao, Ying ² ; Chen, Anhui ² ; Li, Wanzhen ³ ; Wang, Jiuxiang ¹ ; Wang, Yulong ³ ;

作者机构： 1.Anhui Univ Chinese Med, Affiliated Hosp 1, Expt Ctr Clin Res, Hefei, Anhui, Peoples R China

2.Xuzhou Univ Technol, Jiangsu Key Construct Lab Food Resource Dev & Qua, Xuzhou, Jiangsu, Peoples R China

3.Anhui Agr Univ, Anhui Prov Key Lab Microbial Pest Control, Hefei 230036, Anhui, Peoples R China

4.Anhui Polytech Univ, Engn Technol Res Ctr Microbial Fermentat Anhui Pr, Wuhu, Peoples R China

5.Anhui Acad Agr Sci, Key Lab Crop Qual Improvement Anhui Prov, Crop Res Inst, Hefei, Anhui, Peoples R China

关键词： pest fungal pathogen; fungal pathogenesis; RNA-seq; infection strategies; virulence genes

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期： 2019 年 75 卷 5 期

页码：

收录情况： SCI

摘要： BACKGROUND Insect pests have evolved various defense mechanisms to combat fungal infection, and fungi have developed multiple strategies to overcome the immune defense responses of insects. However, transcriptomic analysis of fungal strategies for infecting different pests has not been reported. RESULTS Transcriptomic profiling of Beauveria bassiana was performed at 12, 24 and 48 h after infecting Galleria mellonella and Plutella xylostella, and 540, 847 and 932 differentially expressed genes were detected, respectively. Functional categorization showed that most of these genes are involved in the ribosome, nitrogen metabolism and oxidative phosphorylation pathways. Thirty-one differentially expressed virulence genes (including genes involved in adhesion, degradation, host colonization and killing, and secondary metabolism) were found, suggesting that different molecular mechanisms were used by the fungus during the infection of different pests, which was further confirmed by disrupting creA and fkh2. Virulence assay results showed that Delta creA and Delta fkh2 strains of B. bassiana had distinct fold changes in their 50% lethal time (LT50) values (compared with the control stains) during infection of G. mellonella (Delta creA: 1.38-fold > Delta fkh2: 1.18-fold) and P. xylostella (Delta creA: 1.44-fold Delta fkh2: 2.25-fold). creA was expressed at higher levels during the infection of G. mellonella compared with P. xylostella, whereas fkh2 showed the opposite expression pattern, demonstrating that creA and Fkh2 have different roles in B. bassiana during the infection of G. mellonella and P. xylostella. CONCLUSION These findings demonstrate that B. bassiana regulates different genes to infect different insects, advancing knowledge of the molecular mechanisms of Beauveria-pest interactions. (c) 2018 Society of Chemical Industry