milR4 and milR16 Mediated Fruiting Body Development in the Medicinal Fungus Cordyceps militaris
文献类型： 外文期刊
作者： Shao, Ying 1 ; Tang, Jin 2 ; Chen, Shanglong 1 ; Wu, Yonghua 1 ; Wang, Kun 3 ; Ma, Bin 3 ; Zhou, Qiumei 4 ; Chen, Anhui 1 ;
作者机构： 1.Xuzhou Univ Technol, Jiangsu Key Lab Food Resource Dev & Qual Safe, Xuzhou, Jiangsu, Peoples R China
2.Jiangsu Xuzhou Technician Inst, Xuzhou, Jiangsu, Peoples R China
3.Jiangsu KONEN Biol Engn Co Ltd, Nanjing, Jiangsu, Peoples R China
4.Anhui Univ Chinese Med, Expt Ctr Clin Res, Affiliated Hosp 1, Hefei, Anhui, Peoples R China
5.Anhui Agr Univ, Anhui Prov Key Lab Microbial Pest Control, Hefei, Anhui, Peoples R China
6.Anhui Acad Agr Sci, Key Lab Crop Qual Improvement Anhui Prov, Crop Res Inst, Hefei, Anhui, Peoples R China
关键词： microRNA-like RNAs (milRNAs); sexual development; Cordyceps militaris; disruption; over-expression
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2019 年 10 卷
页码：
收录情况： SCI
摘要： Cordyceps militaris readily performs sexual reproduction, thus providing a remarkably rich model for understanding the processes involved in sexual development. It could regulate expression of human genes by diet-derived miRNA-like RNAs (milRNAs). However, the study of miRNAs in C. militaris has been limited. In the present study, genes encoding Dicers, Argonautes, and RNA-dependent RNA polymerases were identified. Illumina deep sequencing was performed to characterize the milRNAs in C. militaris at asexual and sexual development stages. Total 38 milRNAs were identified and five milRNAs were validated by northern blot and qRT-PCR, out of which, 19 were specific for sexual development. Importantly, the fungi could not form fruiting bodies after disruption of milR4, while the perithecium was formed in advance after over-expression of milR4. Abnormal pale yellow fruiting body primordium, covered with abnormal primordium, was formed in the strain with miR16 disruption. Although no milR4 or milR16 target genes were identified, differential expression of many different genes involved in mycelium growth and sexual development (mating process, mating signaling, and fruiting body development) among these mutants were found. Overall, milRNAs play vital roles in sexual development in C. militaris.
- 相关文献
作者其他论文 更多>>
-
YqeH contributes to avian pathogenic Escherichia coli pathogenicity by regulating motility, biofilm formation, and virulence
作者：Yin, Lei;Pan, Xiaocheng;Cheng, Baoyan;Tu, Jian;Shao, Ying;Song, Xiangjun;Qi, Kezong
关键词：Avian pathogenic Escherichia coli; yqeH; motility; biofilm formation; virulence; pathogenicity
-
The role of Escherichia coli type III secretion system 2 chaperone protein ygeG in pathogenesis of avian pathogenic Escherichia coli
作者：Yin, Lei;Wang, Zeping;Pan, Xiaocheng;Li, Qianwen;Wang, Zeping;Tu, Jian;Shao, Ying;Song, Xiangjun;Qi, Kezong
关键词：Avian pathogenic Escherichia coli; ETT2; Chaperone protein; ygeG; Pathogenesis; Virulence
-
The Escherichia coli type III secretion system 2 Is involved in the biofilm formation and virulence of avian Pathogenic Escherichia coli
作者：Yin, Lei;Li, Qianwen;Wang, Zeping;Tu, Jian;Shao, Ying;Song, Xiangjun;Qi, Kezong;Yin, Lei;Shen, Xuehuai;Pan, Xiaocheng
关键词：Avian pathogenic Escherichia coli; ETT2; Biofilm formation; Virulence; Pathogenesis; RNA sequencing
-
Fowl adenovirus serotype 4 uses gga-miR-181a-5p expression to facilitate viral replication via targeting of STING
作者：Yin, Dongdong;Shao, Ying;Yang, Kankan;Tu, Jian;Song, Xiangjun;Qi, Kezong;Yin, Dongdong;Pan, Xiaocheng
关键词：Fowl adenovirus serotype 4; gga-miR-181a-5p; STING; Viral replication
-
In vivo transcriptomic analysis of Beauveria bassiana reveals differences in infection strategies in Galleria mellonella and Plutella xylostella
作者：Zhou, Qiumei;Wang, Jiuxiang;Shao, Ying;Chen, Anhui;Li, Wanzhen;Wang, Yulong;Li, Wanzhen;Wang, Yulong
关键词：pest fungal pathogen; fungal pathogenesis; RNA-seq; infection strategies; virulence genes
-
Whole-genome resequencing reveals world-wide ancestry and adaptive introgression events of domesticated cattle in East Asia
作者：Chen, Ningbo;Cai, Yudong;Chen, Qiuming;Li, Ran;Huang, Yongzhen;Huang, Shisheng;Zheng, Zhuqing;Ma, Zhijie;Dang, Ruihua;Lan, Xianyong;Chen, Hong;Jiang, Yu;Le, Chuzhao;Wang, Kun;Hu, Songmei;Sun, Zhouyong;Zhang, Hucai;Song, Weining;Ma, Zhijie;Ma, Yun;Zhang, Zijing;Xu, Lei;Jia, Yutang;Liu, Shanzhai;Yue, Xiangpeng;Deng, Weidong;Zhang, Xiaoming;Han, Jianlin;Han, Jianlin;Bradley, Daniel G.
关键词：
