Purification, characterization and immunomodulatory activity of polysaccharides from stem lettuce
文献类型： 外文期刊
作者： Nie, Chenzhipeng 1 ; Zhu, Peilei 1 ; Ma, Shuping 1 ; Wang, Mingchun 1 ; Hu, Youdong 4 ;
作者机构： 1.Anhui Agr Univ, Anhui Prov Engn Lab Agr Prod Proc, Hefei 230036, Peoples R China
2.Anhui Agr Univ, Dept Food Sci & Engn, Hefei 230036, Anhui, Peoples R China
3.Anhui Acad Agr Sci, Inst Hort, Hefei 230031, Peoples R China
4.Xuzhou Med Coll, Affiliated Huaian Hosp, Huaian 223002, Peoples R China
关键词： Polysaccharide; Stem lettuce; Purification; Characterization; Immunomodulatory
期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）
ISSN： 0144-8617
年卷期： 2018 年 188 卷
页码：
收录情况： SCI
摘要： Stem lettuce has a long history of cultivation in China and possesses high nutritional and medicinal value. In our previous studies, extraction optimization, characterization, and bioactivities of stem lettuce polysaccharides (SLP) were investigated. In this study, SLP were further separated into two purified polysaccharides, SLP-1 and SLP-2, by anion exchange chromatography followed by size exclusion chromatography. SLP-1, with a molecular weight of 90 KDa, was mainly composed of galacturonic acid, galactose and arabinose in a molar ratio of 17.6:41.7:33.9. SLP-2, with a molecular weight of 44 KDa, was mainly composed of mannose, galacturonic acid, galactose and arabinose in a molar ratio of 11.5:69.5:9.3:8.2. In addition, both purified polysaccharides contain sulphate radicals, have triple helical structures and can promote macrophage proliferation without cytotoxicity. SLP-2 was better able to stimulate phagocytic and nitric oxide production than SLP-1. The results suggest that polysaccharides from stem lettuce could be explored as immunomodulatory agents in the field of pharmaceuticals and functional foods.
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