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Integration of miRNAs, Degradome, and Transcriptome Omics Uncovers a Complex Regulatory Network and Provides Insights Into Lipid and Fatty Acid Synthesis During Sesame Seed Development

文献类型： 外文期刊

作者： Zhang, Yin-Ping 1 ; Zhang, Yuan-Yuan 2 ; Thakur, Kiran 2 ; Zhang, Fan 2 ; Hu, Fei 2 ; Zhang, Jian-Guo 2 ; Wei, Peng-Ch 1 ;

作者机构： 1.Anhui Acad Agr Sci, Crop Res Inst, Hefei, Peoples R China

2.Hefei Univ Technol, Sch Food & Biol Engn, Hefei, Peoples R China

3.Anhui Agr Univ, Coll Agron, Hefei, Peoples R China

4.Anhui Acad Agr Sci, Rice Res Inst, Key Lab Rice Genet Breeding Anhui Prov, Hefei, Peoples R China

关键词： sesame; lipid fatty acid; biosynthesis; network biology; seed development

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.754； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2021 年 12 卷

页码：

收录情况： SCI

摘要： Sesame (Sesamum indicum L.) has always been known as a health-promoting oilseed crop because of its nutrient-rich oil. In recent years, studies have focused on lipid and fatty acid (FA) biosynthesis in various plants by high-throughput sequencing. Here, we integrated transcriptomics, small RNAs, and the degradome to establish a comprehensive reserve intensive on key regulatory micro RNA (miRNA)-targeting circuits to better understand the transcriptional and translational regulation of the oil biosynthesis mechanism in sesame seed development. Deep sequencing was performed to differentially express 220 miRNAs, including 65 novel miRNAs, in different developmental periods of seeds. GO and integrated KEGG analysis revealed 32 pairs of miRNA targets with negatively correlated expression profiles, of which 12 miRNA-target pairs were further confirmed by RT-PCR. In addition, a regulatory co-expression network was constructed based on the differentially expressed gene (DEG) profiles. The FAD2, LOC10515945, LOC105161564, and LOC105162196 genes were clustered into groups that regulate the accumulation of unsaturated fatty acid (UFA) biosynthesis. The results provide a unique advanced molecular platform for the study of lipid and FA biosynthesis, and this study may serve as a new theoretical reference to obtain increased levels of UFA from higher-quality sesame seed cultivars and other plants.

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