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The fowl adenovirus serotype 4 (FAdV-4) induce cellular pathway in chickens to produce interferon and antigen-presented molecules (MHCI/II)

文献类型： 外文期刊

作者： Li, Meizhen 1 ; Raheem, Muhammad Akmal 1 ; Han, Chunyang 1 ; Yu, Fengmei 1 ; Dai, Yin 2 ; Imran, Muhammad 3 ; Hong, Qi 1 ;

作者机构： 1.Anhui Agr Univ, Coll Anim Sci & Technol, Key Lab Vet Pathobiol & Dis Control, Hefei 230036, Anhui, Peoples R China

2.Anhui Acad Agr Sci, Hefei 230036, Anhui, Peoples R China

3.Univ Vet & Anim Sci, Dept Vet Surg & Pet Sci, Lahore 54000, Punjab, Pakistan

关键词： cytokines; pathogenic; interferon; MHC; transcription factor

期刊名称：POULTRY SCIENCE （ 影响因子：3.352； 五年影响因子：3.679 ）

ISSN：

年卷期： 2021 年 100 卷 10 期

页码：

收录情况： SCI

摘要： FAdV-4 is the major strain of adenovirus that responsible for hydro-pericardial syndrome (HPS) in poultry. In this study, the virus's specific gene frag-ments were isolated from clinically suspected cases and amplified by PCR. Finally, after a viral infection to investigate the immune response of the host, the gene expression of MHC (major histo-compatible) molecules (MHCI alpha, MHCII beta), Ii (Invariant Chain) gene, inflam-matory cytokines (IFN-beta, IFN-gamma, and IL-1 beta), and tran-scription factors (MDA5, STING, IRF7, and NF-kappa B) were detected by real-time PCR (fluorescence technol-ogy). The results of sequence comparison showed that the clinically isolated virus was 100% homologous to a virulent strain of avian adenovirus group C serotype 4 (FAdV-4), which were named AH-FAdV-4. The TCID50 and pathogenicity of the virus were determined that was 10(6.52)/0.1 mL with a mortality rate of 100% in chickens and 0% in ducks. Furthermore, results showed that the expression level of MHCIa, MHCII beta, and Ii genes in chicken embryo kidney cells significantly (P < 0.01) upregulated (increased) after infection, which was 43, 5.2, and 2.5 times higher than the control group. With the addition of PDTC, an inhibitor of NF-kappa B, then the expression level of MHCI alpha, MHCII beta, and Ii was decreased significantly (P < 0.01) than the control group. The transcription levels of these genes were decreased 0.64, 0.27, and 0.26 respectively. Simultaneously, the expression levels of IFN-beta, IFN-gamma, and IL-1 beta were also significantly (P < 0.01) up-regulated (increased) 7.8, 22.7, and 5 times higher than the control group. It was found that up-regulation of STING and NF-kappa B path-ways are directly involved in the regulation of inflamma-tory cytokines (IFN-beta, IFN-gamma, and IL-1 beta), MHC molecules (MHCI alpha, MHCII beta), and Ii gene. The results also showed that the gene regulation pathways consecu-tively increased the expression levels of MDA5, STING, IRF7, and NF-kappa B. It is conducted that the expression levels of cytokines, MHC molecules, and li gene were increased by STING and NF-kappa B pathways.

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